Mouse model of chronic and binge ethanol feeding (the NIAAA model)

被引：938
作者：
Bertola, Adeline [1 ]
Mathews, Stephanie [1 ]
Ki, Sung Hwan [1 ]
Wang, Hua [1 ]
Gao, Bin [1 ]
机构：
[1] NIAAA, Lab Liver Dis, NIH, Bethesda, MD USA
来源：
NATURE PROTOCOLS | 2013年 / 8卷 / 03期
关键词：
ALCOHOLIC LIVER-DISEASE; OXIDATIVE STRESS; INTRAGASTRIC INFUSION; HEPATIC STEATOSIS; FATTY LIVER; TNF-ALPHA; FED MICE; INJURY; PATHOGENESIS; STEATOHEPATITIS;
D O I：
10.1038/nprot.2013.032
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Chronic alcohol consumption is a leading cause of chronic liver disease worldwide, leading to cirrhosis and hepatocellular carcinoma. Currently, the most widely used model for alcoholic liver injury is ad libitum feeding with the Lieber-DeCarli liquid diet containing ethanol for 4-6 weeks; however, this model, without the addition of a secondary insult, only induces mild steatosis, slight elevation of serum alanine transaminase (ALT) and little or no inflammation. Here we describe a simple mouse model of alcoholic liver injury by chronic ethanol feeding (10-d ad libitum oral feeding with the Lieber-DeCarli ethanol liquid diet) plus a single binge ethanol feeding. This protocol for chronic-plus-single-binge ethanol feeding synergistically induces liver injury, inflammation and fatty liver, which mimics acute-on-chronic alcoholic liver injury in patients. This feeding protocol can also be extended to chronic feeding for longer periods of time up to 8 weeks plus single or multiple binges. Chronic-binge ethanol feeding leads to high blood alcohol levels; thus, this simple model will be very useful for the study of alcoholic liver disease (ALD) and of other organs damaged by alcohol consumption.
引用
收藏
页码：627 / 637
页数：11
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