Visual Detection of Potato leafroll virus by One-step Reverse Transcription Loop-Mediated Isothermal Amplification of DNA with Hydroxynaphthol Blue Dye

被引：27

作者：

Ahmadi, Sayvan ^{[1
]}

Almasi, Mohammad A. ^{[2
]}

Fatehi, Foad ^{[1
]}

Struik, Paul C. ^{[3
]}

Moradi, Aboubakr ^{[2
]}

机构：

[1] Univ Tehran, Dept Agron & Plant Breeding, Karaj, Iran

[2] Univ Zanjan, Dept Plant Biotechnol, Coll Agr, Zanjan, Iran

[3] Wageningen Univ, Crop & Weed Ecol Grp, Dept Plant Sci, NL-6700 AK Wageningen, Netherlands

来源：

JOURNAL OF PHYTOPATHOLOGY | 2013年 / 161卷 / 02期

关键词：

Potato leafroll virus; one-step RT-LAMP; hydroxynaphthol blue dye; NUCLEIC-ACID AMPLIFICATION; RAPID DETECTION; LAMP; TUBERS; ASSAY; SPP;

D O I：

10.1111/jph.12037

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Loop-mediated isothermal amplification (LAMP) assay is a novel technique for amplifying DNA under constant temperature, with high specificity, sensitivity, rapidity and efficiency. We applied reverse transcription loop-mediated isothermal amplification (RT-LAMP) to visually detect Potato leafroll virus. One-step RT-LAMP was performed using RNA of PLRV-infected potato leaves and a set of primers (F3, B3, FIP, BIP, LF and LB) designed for RT-LAMP reaction of the coat protein (CP) gene of PLRV. Positive effects of RT-LAMP were detected by agarose gel electrophoresis and hydroxynaphthol blue (HNB) dye and were shown by a colour change from violet to sky blue. RT-LAMP with HNB dye proved to be a simple assay for the rapid detection of PLRV.

引用

页码：120 / 124

页数：5

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