Elastic and Conformational Softness of a Globular Protein

被引：52

作者：

Hong, Liang ^{[1
]}

Glass, Dennis C. ^{[1
,2
]}

Nickels, Jonathan D. ^{[3
]}

Perticaroli, Stefania ^{[3
]}

Yi, Zheng ^{[1
]}

Madhusudan, Tyagi ^{[4
,5
]}

O'Neill, Hugh ^{[6
,7
]}

Zhang, Qiu ^{[6
]}

Sokolov, Alexei P. ^{[3
]}

Smith, Jeremy C. ^{[1
,7
]}

机构：

[1] Oak Ridge Natl Lab, UT ORNL Ctr Mol Biophys, Oak Ridge, TN 37831 USA

[2] Univ Tennessee, Grad Sch Genome Sci & Technol, Knoxville, TN 37996 USA

[3] Univ Tennessee, Oak Ridge Natl Lab, Joint Inst Neutron Sci, Dept Chem, Oak Ridge, TN 37831 USA

[4] NIST, Ctr Neutron Res, Gaithersburg, MD 20899 USA

[5] Univ Maryland, Dept Mat Sci & Engn, College Pk, MD 20742 USA

[6] Oak Ridge Natl Lab, Biol & Soft Matter Div, Oak Ridge, TN 37931 USA

[7] Univ Tennessee, Dept Biochem & Cellular & Mol Biol, Knoxville, TN 37996 USA

来源：

PHYSICAL REVIEW LETTERS | 2013年 / 110卷 / 02期

基金：

美国国家科学基金会;

关键词：

INELASTIC NEUTRON-SCATTERING; DYNAMICAL TRANSITION; HYDRATION WATER; SIDE-CHAIN; MOTIONS; TEMPERATURE; MYOGLOBIN; LYSOZYME;

D O I：

10.1103/PhysRevLett.110.028104

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Flexibility, or softness, is crucial for protein function and consists of a conformational component, involving jumps between potential wells, and an elastic component, involving fluctuations within the wells. Combining molecular dynamics simulation with incoherent neutron scattering and light scattering measurements on green fluorescent protein, we reveal a relationship between the intrawell fluctuations and elastic moduli of the protein. This finding leads to a simple means of experimentally separating the conformational from the elastic atomic displacements. DOI: 10.1103/PhysRevLett.110.028104

引用

页数：5

共 30 条

[1]

[Anonymous], [No title captured]

[2] Energy resolution and dynamical heterogeneity effects on elastic incoherent neutron scattering from molecular systems [J].

Becker, T ;

Smith, JC .

PHYSICAL REVIEW E, 2003, 67 (02) :8-219048

[3] Protein flexibility from the dynamical transition: A force constant analysis [J].

Bicout, DJ ;

Zaccai, G .

BIOPHYSICAL JOURNAL, 2001, 80 (03) :1115-1123

[4] Protein and solvent dynamics: How strongly are they coupled? [J].

Caliskan, G ;

Mechtani, D ;

Roh, JH ;

Kisliuk, A ;

Sokolov, AP ;

Azzam, S ;

Cicerone, MT ;

Lin-Gibson, S ;

Peral, I .

JOURNAL OF CHEMICAL PHYSICS, 2004, 121 (04) :1978-1983

[5] Observation of fragile-to-strong dynamic crossover in protein hydration water [J].

Chen, S. -H. ;

Liu, L. ;

Fratini, E. ;

Baglioni, Piero ;

Faraone, A. ;

Mamontov, E. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (24) :9012-9016

[6] DYNAMICAL TRANSITION OF MYOGLOBIN REVEALED BY INELASTIC NEUTRON-SCATTERING [J].

DOSTER, W ;

CUSACK, S ;

PETRY, W .

NATURE, 1989, 337 (6209) :754-756

[7] MULTIPLE CONFORMATIONAL STATES OF PROTEINS - A MOLECULAR-DYNAMICS ANALYSIS OF MYOGLOBIN [J].

ELBER, R ;

KARPLUS, M .

SCIENCE, 1987, 235 (4786) :318-321

[8] CORRELATED INTRAMOLECULAR MOTIONS AND DIFFUSE-X-RAY SCATTERING IN LYSOZYME [J].

FAURE, P ;

MICU, A ;

PERAHIA, D ;

DOUCET, J ;

SMITH, JC ;

BENOIT, JP .

NATURE STRUCTURAL BIOLOGY, 1994, 1 (02) :124-128

[9] THERMAL MOTIONS AND FUNCTION OF BACTERIORHODOPSIN IN PURPLE MEMBRANES - EFFECTS OF TEMPERATURE AND HYDRATION STUDIED BY NEUTRON-SCATTERING [J].

FERRAND, M ;

DIANOUX, AJ ;

PETRY, W ;

ZACCAI, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9668-9672

[10] THE ENERGY LANDSCAPES AND MOTIONS OF PROTEINS [J].

FRAUENFELDER, H ;

SLIGAR, SG ;

WOLYNES, PG .

SCIENCE, 1991, 254 (5038) :1598-1603

← 1 2 3 →