Hydrogel-encapsulated lipid membranes

被引：92

作者：

Jeon, TJ ^{[1
]}

Malmstadt, N ^{[1
]}

Schmidt, JJ ^{[1
]}

机构：

[1] Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA 90095 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 01期

关键词：

D O I：

10.1021/ja056901v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have encapsulated free-standing planar lipid membranes in a polymer hydrogel via in situ photopolymerization. These encapsulated membranes were measured to last at least 5 days and provided a stable environment for single-molecule measurements of incorporated α-hemolysin channel proteins. The translocation of single-stranded DNA through α-hemolysin incorporated into these encapsulated membranes is slowed over 100 times relative to that of the gel-free state. These stabilized membranes, therefore, have application to the single-molecule analysis of DNA as well as broader application to biomolecular sensors, membrane protein biophysical studies, and drug discovery. Copyright © 2006 American Chemical Society.

引用

页码：42 / 43

页数：2

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