Autofocusing of digital holographic microscopy based on off-axis illuminations

被引:67
作者
Gao, Peng [1 ]
Yao, Baoli [1 ]
Min, Junwei [1 ]
Guo, Rongli [1 ]
Ma, Baiheng [1 ]
Zheng, Juanjuan [1 ]
Lei, Ming [1 ]
Yan, Shaohui [1 ]
Dan, Dan [1 ]
Ye, Tong [1 ]
机构
[1] Chinese Acad Sci, State Key Lab Transient Opt & Photon, Xian Inst Opt & Precis Mech, Xian 710119, Peoples R China
关键词
NUMERICAL RECONSTRUCTION; PHASE MICROSCOPY; INTERFEROMETRY; DIFFRACTION; OBJECTS; FIELD;
D O I
10.1364/OL.37.003630
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
An auto-focusing method for digital holographic microscopy has been proposed by employing two off-axis illumination beams. When specimens are illuminated by two plane waves in different directions, it is found that the farther the reconstruction plane is from the image plane, the wider the two reconstructed images are separated from each other. Thus, the image plane can be determinated by finding the minimum of the variation between the two reconstructed object waves on both the amplitude and phase distributions. The feasibility of the proposed method is demonstrated by the corresponding simulation and experiment. (c) 2012 Optical Society of America
引用
收藏
页码:3630 / 3632
页数:3
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