Colony to colorimetry in 6 h: ELISA detection of a surface-expressed Pseudomonas aeruginosa virulence factor using immobilized bacteria

被引：1

作者：

Adawi, Azmi ^{[1
]}

Neville, Lewis F. ^{[1
]}

机构：

[1] Lostam BioPharmaceut Ltd, IL-17111 Nazareth, Israel

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2012年 / 74卷 / 01期

关键词：

Pseudomonas aeruginosa (PA); Flagellin; Monoclonal antibody (mAb); Multidrug resistant (MDR); Fixed ELISA; MONOCLONAL-ANTIBODIES; PNEUMONIA; INFECTION; FLAGELLIN; EPIDEMIOLOGY; CHALLENGE; INDUCE;

D O I：

10.1016/j.diagmicrobio.2012.05.016

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A rapid ELISA employing intact Pseudomonas aeruginosa (PA) is described that allows discrimination between strains harboring flagellin type a or b. All 52 PA strains known to harbor flagellin type b were positive in this ELISA when screened with a fully human monoclonal antibody (LST-007) targeting flagellin type b. Completion of this assay in only 6 h, from picking a single bacterial colony to a colorimetric product, could easily be adapted to a clinical laboratory setting and permit the appropriate choice of therapeutic monoclonal antibody versus its homologous flagellin target in PA-infected patients. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：84 / 87

页数：4