Positional cloning of rice semidwarfing gene, sd-1:: Rice "Green revolution gene" encodes a mutant enzyme involved in gibberellin synthesis

被引：421

作者：

Monna, L ^{[1
]}

Kitazawa, N ^{[1
]}

Yoshino, R ^{[1
]}

Suzuki, J ^{[1
]}

Masuda, H ^{[1
]}

Maehara, Y ^{[1
]}

Tanji, M ^{[1
]}

Sato, M ^{[1
]}

Nasu, S ^{[1
]}

Minobe, Y ^{[1
]}

机构：

[1] Plant Genome Ctr, Tsukuba, Ibaraki 3050856, Japan

来源：

DNA RESEARCH | 2002年 / 9卷 / 01期

关键词：

rice (Oryza sativa L.); positional cloning; sd-1; cleaved amplified polymorphic sequence (CAPS); single nucleotide polymorphism (SNP);

D O I：

10.1093/dnares/9.1.11

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A rice semidwarfing gene, sd-1, known as the "green revolution gene," was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice cultivars have an identical sequence in this region, consisting of 3 exons (558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the expressed sequence), from the middle of exon 1 to upstream of exon 2, including a 105-bp intron, resulting in a frame-shift that produces a termination codon after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe [TTC]). Expression analysis suggests the existence of at least one more locus of gibberellin 20-oxidase which may prevent severe dwarfism from developing in sd-1 mutants.

引用

页码：11 / 17

页数：7

共 22 条

[1] High-resolution mapping and isolation of a yeast artificial chromosome contig containing fw2.2: A major fruit weight quantitative trait locus in tomato [J].