TOX3 regulates calcium-dependent transcription in neurons

被引：64

作者：

Yuan, Shauna H. ^{[1
]}

Qiu, Zilong ^{[2
]}

Ghosh, Anirvan ^{[2
]}

机构：

[1] Univ Calif San Diego, Dept Neurosci, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Div Biol Sci, Neurobiol Sect, La Jolla, CA 92093 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 08期

关键词：

CBP; CREB; c-fos; activity; CAM KINASE-IV; SOMATOSTATIN GENE; NUCLEAR-PROTEIN; CREB; CBP; PHOSPHORYLATION; ACTIVATION; EXPRESSION; NUCLEOSOME; SELECTION;

D O I：

10.1073/pnas.0805555106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the cloning and characterization of TOX3, a high mobility group box protein involved in mediating calcium-dependent transcription. TOX3 was identified as a calcium-dependent transactivator using the Transactivator Trap screen. We find that TOX3 interacts with both cAMP response element (CRE)-binding protein (CREB) and CREB-binding protein (CBP), and knockdown of the endogenous TOX3 by RNAi leads to significant reduction of calcium-induced c-fos expression and complete inhibition of calcium activation of the c-fos promoter. The effects of TOX3 on calcium-dependent transcription require the CRE elements. These observations identify TOX3 as an important regulator of calcium-dependent transcription and suggest that TOX3 exerts its effect on CRE-mediated transcription via its association with the CREB-CBP complex.

引用

页码：2909 / 2914

页数：6

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