TOX3 regulates calcium-dependent transcription in neurons

被引：64
作者：
Yuan, Shauna H. [1 ]
Qiu, Zilong [2 ]
Ghosh, Anirvan [2 ]
机构：
[1] Univ Calif San Diego, Dept Neurosci, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Div Biol Sci, Neurobiol Sect, La Jolla, CA 92093 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 08期
关键词：
CBP; CREB; c-fos; activity; CAM KINASE-IV; SOMATOSTATIN GENE; NUCLEAR-PROTEIN; CREB; CBP; PHOSPHORYLATION; ACTIVATION; EXPRESSION; NUCLEOSOME; SELECTION;
D O I：
10.1073/pnas.0805555106
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We report the cloning and characterization of TOX3, a high mobility group box protein involved in mediating calcium-dependent transcription. TOX3 was identified as a calcium-dependent transactivator using the Transactivator Trap screen. We find that TOX3 interacts with both cAMP response element (CRE)-binding protein (CREB) and CREB-binding protein (CBP), and knockdown of the endogenous TOX3 by RNAi leads to significant reduction of calcium-induced c-fos expression and complete inhibition of calcium activation of the c-fos promoter. The effects of TOX3 on calcium-dependent transcription require the CRE elements. These observations identify TOX3 as an important regulator of calcium-dependent transcription and suggest that TOX3 exerts its effect on CRE-mediated transcription via its association with the CREB-CBP complex.
引用
收藏
页码：2909 / 2914
页数：6
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