Viscoelasticity of actin-gelsolin networks in the presence of filamin

被引：26

作者：

Goldmann, WH ^{[1
]}

Tempel, M ^{[1
]}

Sprenger, I ^{[1
]}

Isenberg, G ^{[1
]}

Ezzell, RM ^{[1
]}

机构：

[1] TECH UNIV MUNICH,D-8046 GARCHING,GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1997年 / 246卷 / 02期

关键词：

viscoelasticity; actin network; filamin; gelsolin; rheology;

D O I：

10.1111/j.1432-1033.1997.00373.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cross-linking of actin filaments by filamin by means of frequency-dependent rheology yields an increase in the filament's elasticity and stiffness. Higher cross-linker (filamin) ratios are required for mean actin-filament lengths of 5-6 mu m than for random-length distribution of actin filaments. The loss modulus (i.e. the viscous portion) in the region of the internal-chain dynamics [G''(omega) approximate to omega(alpha)] is influenced by the cross-linking of filaments, and with an increasing molar ratio of filamin/actin a reduction of a is observed. Rheological measurements reveal that actin networks are already formed at the polymerizing stage at a molar ratio of filamin/actin of less than 1:100, and electron micrographs show phase separation of actin/filament networks of low density and of actin/filament bundles.

引用

页码：373 / 379

页数：7

共 37 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] MICROINJECTION OF GELSOLIN INTO LIVING CELLS [J].