Mesoporous silica SBA-16/hydroxyapatite-based composite for ciprofloxacin delivery to bacterial bone infection

被引:17
作者
Andrade, Gracielle Ferreira [1 ,2 ]
Quintao Arantes Faria, Jerusa Araujo [3 ]
Gomes, Dawidson Assis [4 ]
Branco de Barros, Andre Luis [2 ]
Fernandes, Renata Salgado [2 ]
Soares Coelho, Amanda Cristina [5 ]
Takahashi, Jacqueline Aparecida [5 ]
Cunha, Armando da Silva, Jr. [2 ]
Barros de Sousa, Edesia Martins [1 ]
机构
[1] CDTN CNEN, Ctr Dev Nucl Technol, Belo Horizonte, MG, Brazil
[2] Fed Univ Minas Gerais UFMG, Fac Pharm, Belo Horizonte, MG, Brazil
[3] Fed Univ Amazonas UFAM, Inst Biol Sci, Dept Physiol Sci, Manaus, Amazonas, Brazil
[4] Fed Univ Minas Gerais UFMG, Inst Biol Sci, Dept Biochem & Immunol, Belo Horizonte, MG, Brazil
[5] Fed Univ Minas Gerais UFMG, Inst Exact Sci, Dept Chem, Belo Horizonte, MG, Brazil
关键词
Mesoporous silica/hydroxyapatite composite; 3-aminopropyltriethoxysilane-APTES; Ciprofloxacin; Bactericidal activity; Technetium-99m; DRUG-DELIVERY; NANOPARTICLES SYNTHESIS; HYDROXYAPATITE; RELEASE; REGENERATION; SBA-16; CYTOCOMPATIBILITY; SCAFFOLDS; COATINGS; TOOLS;
D O I
10.1007/s10971-017-4557-y
中图分类号
TQ174 [陶瓷工业]; TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
The development of systems that can prevent infections and also ensure bone integration as well as regeneration have been of great interest for pharmaceutical technology. In this study, we show the synthesis of surface-functionalized mesoporous silica (SBA-16) and silica composed of calcium phosphate (SBA-16/HA) particles in order to be applied as efficient drug delivery carriers. The particles were synthesized, functionalized with 3-aminopropyltriethoxysilane (APTES) by post-synthesis grafting and loaded with the osteomyelitis antibiotic agent ciprofloxacin. Moreover, the diethylenetriaminepentacetic acid (DTPA) was anchored in silica-APTES to allow measurements of biological process at molecular and cellular levels. Particles were physicochemically characterized by small angle X-ray scattering (SAXS), elemental analysis (CHN), thermogravimetric analysis (TGA), N-2 adsorption and zeta potential analysis. Functionalized silica particles were radiolabeled with technetium-99m showing high radiochemical yields and high radiolabeled stability. In vivo experiments results showed higher bone uptake of the SBA-16/HAAPTES than SBA-16APTES. In addition, bactericidal efficacy of these particles was tested against microorganisms present in bone infection, and our composites had bactericidal efficiency comparable to free-ciprofloxacin. In summary, taking into account the great potential of these silica mesoporous and nanocomposite structures to carry molecules, besides their bactericidal efficacy, these materials are promising candidates for bone infection treatment. [GRAPHICS] .
引用
收藏
页码:369 / 381
页数:13
相关论文
共 35 条
  • [1] Antibiotic resistance and its cost: is it possible to reverse resistance?
    Andersson, Dan I.
    Hughes, Diarmaid
    [J]. NATURE REVIEWS MICROBIOLOGY, 2010, 8 (04) : 260 - 271
  • [2] Osteogenic differentiation of adipose-derived stem cells in mesoporous SBA-16 and SBA-16 hydroxyapatite scaffolds
    Andrade, Gracielle F.
    Carvalho, Juliana L.
    Armando, S. C. Junior
    Goes, Alfredo M.
    Sousa, Edesia M. B.
    [J]. RSC ADVANCES, 2015, 5 (67) : 54551 - 54562
  • [3] An in situ synthesis of mesoporous SBA-16/hydroxyapatite for ciprofloxacin release: in vitro stability and cytocompatibility studies
    Andrade, Gracielle Ferreira
    Gomide, Viviane Silva
    da Silva Junior, Armando Cunha
    Goes, Alfredo Miranda
    Barros de Sousa, Edesia Martins
    [J]. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2014, 25 (11) : 2527 - 2540
  • [4] Mesoporous silica SBA-16 nanoparticles: Synthesis, physicochemical characterization, release profile, and in vitro cytocompatibility studies
    Andrade, Gracielle Ferreira
    Ferreira Soares, Daniel Cristian
    dos Santos, Raquel Gouvea
    Barros Sousa, Edesia Martins
    [J]. MICROPOROUS AND MESOPOROUS MATERIALS, 2013, 168 : 102 - 110
  • [5] [Anonymous], BONE TISSUE REGEN IN
  • [6] Sol-gel silica-based biomaterials and bone tissue regeneration
    Arcos, Daniel
    Vallet-Regi, Maria
    [J]. ACTA BIOMATERIALIA, 2010, 6 (08) : 2874 - 2888
  • [7] Determination of pKa values of active pharmaceutical ingredients
    Babic, Sandra
    Horvat, Alka J. M.
    Pavlovic, Dragana Mutavdzic
    Kastelan-Macan, Marija
    [J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2007, 26 (11) : 1043 - 1061
  • [8] Bancos S., 2012, ISRN Nanotechnology, DOI 10.5402/2012/454072
  • [9] A NEW FAMILY OF MESOPOROUS MOLECULAR-SIEVES PREPARED WITH LIQUID-CRYSTAL TEMPLATES
    BECK, JS
    VARTULI, JC
    ROTH, WJ
    LEONOWICZ, ME
    KRESGE, CT
    SCHMITT, KD
    CHU, CTW
    OLSON, DH
    SHEPPARD, EW
    MCCULLEN, SB
    HIGGINS, JB
    SCHLENKER, JL
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (27) : 10834 - 10843
  • [10] Berridge MV, 2005, BIOTECHNOL ANN REV, V11, P127, DOI 10.1016/S1387-2656(05)11004-7