Reverse transcription-based loop-mediated isothermal amplification strategy for real-time miRNA detection with phosphorothioated probes

被引：32

作者：

AL-maskri, Abdu Ahmed Abdullah ^{[1
]}

Ye, Jiawei ^{[1
]}

Talap, Jadera ^{[1
]}

Hu, Haihong ^{[1
]}

Sun, Lianli ^{[1
]}

Yu, Lushan ^{[1
]}

Cai, Sheng ^{[1
]}

Zeng, Su ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Drug Metab & Pharmaceut Anal, Coll Pharmaceut Sci, Zhejiang Prov Key Lab Anticanc Drug Res, Hangzhou 310058, Zhejiang, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2020年 / 1126卷 / 1126期

基金：

中国国家自然科学基金;

关键词：

Loop-mediated isothermal amplification; miRNA detection; Phosphorothioated modifications; Self-priming; Terminal hairpin; ROLLING-CIRCLE AMPLIFICATION; STRAND DISPLACEMENT AMPLIFICATION; ULTRASENSITIVE DETECTION; ELECTROCHEMICAL BIOSENSOR; MICRORNA; PLATFORM; LAMP; SENSITIVITY; LIGATION; EXCHANGE;

D O I：

10.1016/j.aca.2020.06.007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel reverse transcription-based loop-mediated isothermal amplification (LAMP) strategy for miRNA detection has been developed. This method consists of two stem-loop probes inspired by the dumbbell-shaped amplicons and inner primers used in conventional LAMP reactions. Termed "terminal hairpin formation and self-priming" (THSP), this reaction incorporates phosphorothioated (PS) modifications to achieve DNA folding and extension without primers. The final signal is monitored by a sequence-specific detection probe, which minimizes the background noise. We suggest that our rapid, facile, and reliable LAMP method will be a promising candidate for detecting miRNA in biomedical applications. (C) 2020 Elsevier B.V. All rights reserved.

引用

页码：1 / 6

页数：6

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