Microtubule Plus End Dynamics - Do We Know How Microtubules Grow? Cells boost microtubule growth by promoting distinct structural transitions at growing microtubule ends

被引:20
作者
van Haren, Jeffrey [1 ]
Wittmann, Torsten [1 ]
机构
[1] Univ Calif San Francisco, Dept Cell & Tissue Biol, 513 Parnassus Ave, San Francisco, CA 94143 USA
关键词
CKAP5; cytoskeleton; doublecortin; end-binding proteins; microtubule; microtubule dynamics; ALPHA-BETA-TUBULIN; SLOWLY HYDROLYZABLE ANALOG; GTP HYDROLYSIS; DOUBLECORTIN RECOGNIZES; IN-VITRO; EB1; INSTABILITY; CAP; ORGANIZATION; PROTEINS;
D O I
10.1002/bies.201800194
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microtubules form a highly dynamic filament network in all eukaryotic cells. Individual microtubules grow by tubulin dimer subunit addition and frequently switch between phases of growth and shortening. These unique dynamics are powered by GTP hydrolysis and drive microtubule network remodeling, which is central to eukaryotic cell biology and morphogenesis. Yet, our knowledge of the molecular events at growing microtubule ends remains incomplete. Here, recent ultrastructural, biochemical and cell biological data are integrated to develop a realistic model of growing microtubule ends comprised of structurally distinct but biochemically overlapping zones. Proteins that recognize microtubule lattice conformations associated with specific tubulin guanosine nucleotide states may independently control major structural transitions at growing microtubule ends. A model is proposed in which tubulin dimer addition and subsequent closure of the MT wall are optimized in cells to achieve rapid physiological microtubule growth.
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页数:11
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