Microtubule Plus End Dynamics - Do We Know How Microtubules Grow? Cells boost microtubule growth by promoting distinct structural transitions at growing microtubule ends

被引:20
作者
van Haren, Jeffrey [1 ]
Wittmann, Torsten [1 ]
机构
[1] Univ Calif San Francisco, Dept Cell & Tissue Biol, 513 Parnassus Ave, San Francisco, CA 94143 USA
关键词
CKAP5; cytoskeleton; doublecortin; end-binding proteins; microtubule; microtubule dynamics; ALPHA-BETA-TUBULIN; SLOWLY HYDROLYZABLE ANALOG; GTP HYDROLYSIS; DOUBLECORTIN RECOGNIZES; IN-VITRO; EB1; INSTABILITY; CAP; ORGANIZATION; PROTEINS;
D O I
10.1002/bies.201800194
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microtubules form a highly dynamic filament network in all eukaryotic cells. Individual microtubules grow by tubulin dimer subunit addition and frequently switch between phases of growth and shortening. These unique dynamics are powered by GTP hydrolysis and drive microtubule network remodeling, which is central to eukaryotic cell biology and morphogenesis. Yet, our knowledge of the molecular events at growing microtubule ends remains incomplete. Here, recent ultrastructural, biochemical and cell biological data are integrated to develop a realistic model of growing microtubule ends comprised of structurally distinct but biochemically overlapping zones. Proteins that recognize microtubule lattice conformations associated with specific tubulin guanosine nucleotide states may independently control major structural transitions at growing microtubule ends. A model is proposed in which tubulin dimer addition and subsequent closure of the MT wall are optimized in cells to achieve rapid physiological microtubule growth.
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页数:11
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共 97 条
  • [1] Control of microtubule organization and dynamics: two ends in the limelight
    Akhmanova, Anna
    Steinmetz, Michel O.
    [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2015, 16 (12) : 711 - 726
  • [2] Fission yeast Alp14 is a dose-dependent plus end-tracking microtubule polymerase
    Al-Bassam, Jawdat
    Kim, Hwajin
    Flor-Parra, Ignacio
    Lal, Neeraj
    Velji, Hamida
    Chang, Fred
    [J]. MOLECULAR BIOLOGY OF THE CELL, 2012, 23 (15) : 2878 - 2890
  • [3] High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis
    Alushin, Gregory M.
    Lander, Gabriel C.
    Kellogg, Elizabeth H.
    Zhang, Rui
    Baker, David
    Nogales, Eva
    [J]. CELL, 2014, 157 (05) : 1117 - 1129
  • [4] Microtubule architecture in vitro and in cells revealed by cryo-electron tomography
    Atherton, Joseph
    Stouffer, Melissa
    Francis, Fiona
    Moores, Carolyn A.
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2018, 74 : 572 - 584
  • [5] Self-repair promotes microtubule rescue
    Aumeier, Charlotte
    Schaedel, Laura
    Gaillard, Jeremie
    John, Karin
    Blanchoin, Laurent
    Thery, Manuel
    [J]. NATURE CELL BIOLOGY, 2016, 18 (10) : 1054 - 1064
  • [6] A tethered delivery mechanism explains the catalytic action of a microtubule polymerase
    Ayaz, Pelin
    Munyoki, Sarah
    Geyer, Elisabeth A.
    Piedra, Felipe-Andres
    Vu, Emily S.
    Bromberg, Raquel
    Otwinowski, Zbyszek
    Grishin, Nick V.
    Brautigam, Chad A.
    Rice, Luke M.
    [J]. ELIFE, 2014, 3 : 1 - 19
  • [7] A TOG:αβ-tubulin Complex Structure Reveals Conformation-Based Mechanisms for a Microtubule Polymerase
    Ayaz, Pelin
    Ye, Xuecheng
    Huddleston, Patrick
    Brautigam, Chad A.
    Rice, Luke M.
    [J]. SCIENCE, 2012, 337 (6096) : 857 - 860
  • [8] Doublecortin Recognizes the Longitudinal Curvature of the Microtubule End and Lattice
    Bechstedt, Susanne
    Lu, Kevan
    Brouhard, Gary J.
    [J]. CURRENT BIOLOGY, 2014, 24 (20) : 2366 - 2375
  • [9] Doublecortin Recognizes the 13-Protofilament Microtubule Cooperatively and Tracks Microtubule Ends
    Bechstedt, Susanne
    Brouhard, Gary J.
    [J]. DEVELOPMENTAL CELL, 2012, 23 (01) : 181 - 192
  • [10] Reconstitution of a microtubule plus-end tracking system in vitro
    Bieling, Peter
    Laan, Liedewij
    Schek, Henry
    Munteanu, E. Laura
    Sandblad, Linda
    Dogterom, Marileen
    Brunner, Damian
    Surrey, Thomas
    [J]. NATURE, 2007, 450 (7172) : 1100 - 1105