Actin-driven cell dynamics probed by Fourier transform light scattering

被引：22

作者：

Ding, Huafeng ^{[1
]}

Millet, Larry J. ^{[2
]}

Gillette, Martha U. ^{[2
]}

Popescu, Gabriel ^{[1
]}

机构：

[1] Univ Illinois, Beckman Inst Adv Sci & Technol, Dept Elect & Comp Engn, Quantitat Light Imaging Lab, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Cell & Dev Biol, Urbana, IL 61801 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2010年 / 1卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1364/BOE.1.000260

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We applied the newly developed Fourier transform light scattering (FTLS) to study dynamic light scattering in single live cells, at a temporal scale of seconds to hours. The nanoscale cell fluctuations were measured with and without the active actin contribution. We found experimentally that the spatio-temporal signals rendered by FTLS reveal interesting cytoskeleton dynamics in glial cells (the predominant cell type in the nervous system). The active contribution of actin cytoskeleton was obtained by modulating its dynamic properties via Cytochalasin-D, a drug that inhibits actin polymerization/depolymerization. (C) 2010 Optical Society of America

引用

页码：260 / 267

页数：8

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