Quantification of Major Royal Jelly Protein 1 in Fresh Royal Jelly by Indirect Enzyme-Linked Immunosorbent Assay

被引：12

作者：

Yamaguchi, Kikuji ^{[1
,2
]}

He, Shaoyu ^{[1
,3
]}

Li, Zhengyue ^{[1
]}

Murata, Kiyoshi ^{[4
]}

Hitomi, Nobuyuki ^{[4
]}

Mozumi, Manaho ^{[5
]}

Ariga, Risa ^{[5
]}

Enomoto, Toshiki ^{[5
]}

机构：

[1] Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Yunnan, Peoples R China

[2] Japan Royal Jelly Co Ltd, Head Off, Tokyo 1040031, Japan

[3] Yunnan Agr Univ, Eastern Bee Inst, Kunming 650201, Yunnan, Peoples R China

[4] Japan Royal Jelly Co Ltd, R&D Ctr, Kami, Miyagi 9814263, Japan

[5] Ishikawa Prefectural Univ, Dept Food Sci, Nonoichi, Ishikawa 9218836, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2013年 / 77卷 / 06期

关键词：

royal jelly; MRJP1; apisin; ELISA; quantification; GLYCOPROTEIN APISIN; 57-KDA PROTEIN; PROLIFERATION; PEPTIDES;

D O I：

10.1271/bbb.130013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12-4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.

引用

页码：1310 / 1312

页数：3

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