A novel fluorescent "Turn-Off/Turn-On" system for the detection of acid phosphatase activity

被引：28

作者：

Guo, Pu ^{[1
]}

Yan, Shengyong ^{[1
]}

Zhou, Yimin ^{[1
]}

Wang, Changcheng ^{[1
]}

Xu, Xiaowei ^{[1
]}

Weng, Xiaocheng ^{[1
]}

Zhou, Xiang ^{[1
,2
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Key Lab Biomed Polymers, Minist Educ, Wuhan 430072, Hubei, Peoples R China

[2] Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing, Peoples R China

来源：

ANALYST | 2013年 / 138卷 / 12期

基金：

美国国家科学基金会;

关键词：

ALKALINE-PHOSPHATASE; LABEL-FREE; BIOMARKERS; ASSAY;

D O I：

10.1039/c3an00375b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Acid phosphatase (ACP) can be sensitively, conveniently and efficiently detected via a new fluorescent "Turn-Off/Turn-On" system. Inexpensive (NaPO3)(6) is carefully introduced into this system as a quencher of the aggregation-caused quenching (ACQ) probe. In our method, the limit of detection (LOD) is quite low for detecting ACP, which is an important biomarker and indication of several diseases.

引用

页码：3365 / 3367

页数：3

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