Extracellular matrix-based cryogels for cartilage tissue engineering

被引：54
作者：
Han, Min-Eui [1 ]
Kim, Su-Hwan [1 ]
Kim, Hwan D. [2 ]
Yim, Hyun-Gu [2 ]
Bencherif, Sidi A. [3 ,4 ,6 ]
Kim, Tae-Il [5 ]
Hwang, Nathaniel S. [1 ,2 ]
机构：
[1] Seoul Natl Univ, Interdisciplinary Program Bioengn, Seoul, South Korea
[2] Seoul Natl Univ, Sch Chem & Biol Engn, Inst Chem Proc, N Bio Inst, Seoul, South Korea
[3] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA
[4] Univ Technol Compiegne, UTC CNRS UMR 7338, Biomech & Bioengn BMBI, BP 20529,Rue Personne Roberval, Compiegne, France
[5] Seoul Natl Univ, Sch Dent, Dept Periodontol, Seoul, South Korea
[6] Northeastern Univ, Dept Chem Engn, 360 Huntington Ave, Boston, MA 02215 USA
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 93卷
基金：
新加坡国家研究基金会;
关键词：
Cryogel; Hyaluronic acid; Chondroitin sulfate; Cartilage tissue engineering; Poly (ethylene glycol) diacrylates; CO-PGA HYDROGELS; HYALURONIC-ACID; CHONDROITIN SULFATE; GELATIN CRYOGELS; IN-VITRO; SCAFFOLDS; PROLIFERATION; ATTACHMENT; NETWORKS; DEFECTS;
D O I：
10.1016/j.ijbiomac.2016.05.024
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, we investigated various highly porous extracellular matrix (ECM)-based cryogels for cartilage tissue engineering. For the fabrication of ECM-based cryogels, either methacrylated chondroitin sulfate (MeCS) or methacrylated hyaluronic acid (MeHA) were cross-linked along with poly (ethylene glycol) diacrylates (PEGDA) via free radical polymerization under freezing conditions. This procedure induces ice crystallization (used as a porogen) prior polymer crosslinking in which, after complete cryopolymerization, a thawing process transforms the ice crystals into a unique interconnected macroporous structure within ECM-cryogels. The developed ECM-cryogels exhibited an average macroporosity of 75% and supported the infiltration of chondrocyteds. When rabbit chondrocytes were cultured on ECM-cryogels, MeCS-based cryogels stimulated aggrecan gene expression and GAG accumulation, whereas MeHA-based cryogels stimulated type II collagen gene expression and collagen accumulation. These results demonstrate that design of ECM-based cryogels can play an important role in promoting specific ECM proteins secretion for cartilage tissue engineering. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：1410 / 1419
页数：10
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