RP-HPLC estimation of isradipine in pharmaceutical dosage forms

A reverse phase high performance liquid chromatographic method has been developed for the estimation of isradipine in its capsule dosage forms using RP-C-18 column. The mobile phase (methanol, acetonitrile and acetate buffer. pH 2.8) was pumped at a flow rate of 1 mL/min in the ratio of 60:30: 10 and the eluent were monitored at 290 nm. The intra- and inter-day variation was found to be less than 2% showing high precision of the assay method when compared with reported result. The mean recovery of the drug from the solution containing 200 or 300 mu g/mL was 99.66 +/- 1.20% indicating high precision and accuracy. T e proposed HPLC method may be used for determining isradipine in bulk drug samples or in pharmaceutical dosage forms.