Nitrogen fixation and identification of potential diazotrophs in the Canadian Arctic

被引:100
作者
Blais, Marjolaine [1 ]
Tremblay, Jean-Eric [1 ]
Jungblut, Anne D. [2 ]
Gagnon, Jonathan [1 ]
Martin, Johannie [1 ]
Thaler, Mary [1 ,3 ]
Lovejoy, Connie [1 ,3 ]
机构
[1] Univ Laval, Dept Biol, Quebec City, PQ G1V 0A6, Canada
[2] Univ Laval, Ctr Etud Nordiques, Quebec City, PQ G1V 0A6, Canada
[3] Univ Laval, Inst Biol Integrat & Syst, Quebec City, PQ G1V 0A6, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
OCEANIC FIXED NITROGEN; N-2; FIXATION; DENITRIFICATION RATES; DINITROGEN FIXATION; COMMUNITY STRUCTURE; TROPICAL ATLANTIC; COASTAL WATERS; GENE DIVERSITY; BEAUFORT SEA; DISTRIBUTIONS;
D O I
10.1029/2011GB004096
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Global gaseous nitrogen (N-2) fixation rates may be underestimated and data is lacking from many regions without conspicuous diazotrophic cyanobacteria, such as cold oceans. We estimated N-2 fixation rates at diverse sites in the Canadian Arctic, including the mouth of the Mackenzie River, the offshore Beaufort Sea, Lancaster Sound, Baffin Bay and a river influenced fjord. We also identified potential diazotrophic communities using a targeted survey of the nifH gene. Nitrogen fixation rates ranged from 0.02 nmol N L-1 d(-1) in Baffin Bay to 4.45 nmol N L-1 d(-1) in the Mackenzie River plume. Sequences recovered from the nifH gene survey belonged mainly to Cluster III, a group of nifH sequences associated with diverse microorganisms, with some alpha- and gamma-proteobacteria nifH genes at most sites. Cyanobacteria nifH genes with best matches to Nostocales, which are common in Arctic freshwaters, were recovered from the marine Beaufort Sea. The geographic pattern of N-2 fixation rates and nifH gene identities suggest that the Mackenzie River is the source of a diazotrophic community that contributes new nitrogen to the nitrogen-depleted surface waters of the Beaufort Sea. This first record of N-2 fixation at high latitudes refines our understanding of the global nitrogen budget.
引用
收藏
页数:13
相关论文
共 71 条
[1]   Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques [J].
Aljanabi, SM ;
Martinez, I .
NUCLEIC ACIDS RESEARCH, 1997, 25 (22) :4692-4693
[2]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[3]  
[Anonymous], 2007, SYNTHESIS REPORT
[4]  
[Anonymous], 2005, PHYLIP (phylogeny inference package) version 3.6
[5]   Nitrification rates, ammonium and nitrate distribution in upper layers of the water column and in sediments of the Indian sector of the Southern Ocean [J].
Bianchi, M ;
Feliatra, F ;
Treguer, P ;
Vincendeau, MA ;
Morvan, J .
DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 1997, 44 (05) :1017-1032
[6]   A global marine-fixed nitrogen isotopic budget: Implications for Holocene nitrogen cycling [J].
Brandes, JA ;
Devol, AH .
GLOBAL BIOGEOCHEMICAL CYCLES, 2002, 16 (04)
[7]   Phytoplankton biomass and production in the southeastern Beaufort Sea in autumn 2002 and 2003 [J].
Brugel, Sonia ;
Nozais, Christian ;
Poulin, Michel ;
Tremblay, Jean-Eric ;
Miller, Lisa A. ;
Simpson, Kyle G. ;
Gratton, Yves ;
Demers, Serge .
MARINE ECOLOGY PROGRESS SERIES, 2009, 377 :63-77
[8]  
Carmack EC, 2002, ARCTIC, V55, P29
[9]   Seasonal and spatial patterns of sedimentary denitrification rates in the Chukchi sea [J].
Chang, Bonnie X. ;
Devol, Allan H. .
DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 2009, 56 (17) :1339-1350
[10]   Regional distributions of nitrogen-fixing bacteria in the Pacific Ocean [J].
Church, Matthew J. ;
Bjorkman, Karin M. ;
Karl, David M. ;
Saito, Mak A. ;
Zehr, Jonathan P. .
LIMNOLOGY AND OCEANOGRAPHY, 2008, 53 (01) :63-77