Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan

被引：7

作者：

Bin Kabir, Mohammad Hazzaz ^{[1
,2
]}

Itoh, Megumi ^{[3
]}

Shehata, Ayman Ahmed ^{[4
,5
]}

Bando, Hironori ^{[4
]}

Fukuda, Yasuhiro ^{[4
]}

Murakoshi, Fumi ^{[6
]}

Fujikura, Atsushi ^{[7
]}

Okawa, Hiroaki ^{[7
]}

Endo, Takuto ^{[8
]}

Goto, Akira ^{[9
]}

Kachi, Masayuki ^{[10
]}

Nakayama, Toshie ^{[11
]}

Kano, Yuto ^{[12
]}

Oishi, Shoko ^{[13
]}

Otomaru, Konosuke ^{[13
]}

Essa, Mohamed Ibrahim ^{[5
]}

Kazama, Kei ^{[14
]}

Xuan, Xuenan ^{[1
]}

Kato, Kentaro ^{[1
,4
]}

机构：

[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Inada Cho, Obihiro, Hokkaido 0808555, Japan

[2] Sher E Bangla Agr Univ, Dept Microbiol & Parasitol, Dhaka 1207, Bangladesh

[3] Obihiro Univ Agr & Vet Med, Dept Vet Med, Obihiro, Hokkaido 0808555, Japan

[4] Tohoku Univ, Grad Sch Agr Sci, Lab Sustainable Anim Environm, 232-3 Yomogida, Osaki, Miyagi 9896711, Japan

[5] Zagazig Univ, Fac Vet Med, Dept Anim Med, Infect Dis, Moawwad 44511, Qesm Awel Az Za, Egypt

[6] Kyoto Prefectural Univ Med, Dept Infect Dis, Kamigyo Ku, 465 Kajiicho,Kawaramachi Hirokoji, Kyoto 6028566, Japan

[7] Fukuoka Prefecture Dairy Farming Cooperat, Fukuoka Dairy Cattle Artificial Inseminat Clin, Nishi Ku, 1-13-4 Susenji, Fukuoka, Fukuoka 8390832, Japan

[8] Fukuoka Prefecture Dairy Farming Cooperat, Kurume Dairy Cattle Artificial Inseminat Clin, 75-2 Airaku,Ohashimachi, Kurume, Fukuoka 8390832, Japan

[9] Obihiro Univ Agr & Vet Med, Vet Med Ctr, 2-11 Inada Nishi, Obihiro, Hokkaido 0808555, Japan

[10] Gifu Prefectural Livestock Res Inst, Dairy Res Dept, 1975-615 Kubohara,Yamaoka Cho, Ena, Gifu 5097601, Japan

[11] Miyazaki Agr Mutual Aid Assoc, 17938-5 Nyuta Shintomi Cho, Miyazaki 8891406, Japan

[12] Soo Agr Mutual Aid Assoc, 2253 Osumicho Tsukino, Soo, Kagoshima 8998212, Japan

[13] Kagoshima Univ, Joint Fac Vet Med, 1-21-24 Korimoto, Kagoshima 8900065, Japan

[14] Azabu Univ, Sch Vet Med, Chuou Ku, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 2525201, Japan

来源：

PARASITOLOGY INTERNATIONAL | 2020年 / 78卷

关键词：

Cryptosporidium; C; parvum; Japan; PCR-based sequencing; Phylogenetic analyses; Pre-weaned calves; DEER-LIKE GENOTYPE; PRE-WEANED CALVES; MOLECULAR CHARACTERIZATION; NUCLEOTIDE SUBSTITUTIONS; DAIRY-CATTLE; PARVUM; PREVALENCE; HUMANS; BOVIS; SPP;

D O I：

10.1016/j.parint.2020.102153

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of hosts including humans, and domestic and wild animals. The aim of this study was to molecularly characterize the Cryptosporidium spp. found in calf faeces in Japan. A total of 80 pre-weaned beef and dairy calves' diarrhoeic faecal specimens were collected from nine different prefectures in Japan. A nested polymerase chain reaction targeting the small subunit 18S rRNA and GP60 genes were used to detect the Cryptosporidium genotypes and subtypes. 83.8% (67 out of 80) of the specimens were positive for Cryptosporidium spp.; Cryptosporidium was found in both beef and dairy calves. Cryptosporidium parvum was the predominant species, detected in 77.5% (31/40) of beef calves and 80% (32/40) of dairy calves. Cryptosporidium bovis was also detected, 5.0% (2/40) of dairy calves, and C. ryanae was also found 2.5% (1/40) of dairy calves. One mixed-species infection, 2.5% (1/40) was detected in a beef calf having C. parvum, and C. ryanae. We detected the most common subtype of C. parvum (i.e., IIaA15G2R1), as well as other subtypes (i.e., IIaA14G3R1, IIaA14G2R1, and IIaA13G1R1) that have not previously been detected in calves in Japan. Our results demonstrate the widespread diversity of Cryptosporidium infection in calves in Japan.

引用

页数：7

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