Structural basis for controlling the enzymatic properties of polymannuronate preferred alginate lyase FlAlyA from the PL-7 family

被引：54

作者：

Qin, Hui-Min ^{[1
,2
]}

Miyakawa, Takuya ^{[1
]}

Inoue, Akira ^{[3
]}

Nishiyama, Ryuji ^{[3
]}

Nakamura, Akira ^{[1
]}

Asano, Atsuko ^{[1
]}

Ojima, Takao ^{[3
]}

Tanokura, Masaru ^{[1
,2
]}

机构：

[1] Univ Tokyo, Lab Basic Sci Hlth Longev, Dept Appl Biol Chem, Grad Sch Agr & Life Sci,Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan

[2] Tianjin Univ Sci & Technol, Coll Biotechnol, 29,13th Ave, Tianjin 300457, Peoples R China

[3] Hokkaido Univ, Lab Marine Biotechnol & Microbiol, Grad Sch Fisheries Sci, 3-1-1 Minato Cho, Hakodate, Hokkaido 0418611, Japan

来源：

CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 05期

基金：

中国国家自然科学基金;

关键词：

SUBSTRATE RECOGNITION; MAJOR SOURCES; A1;

D O I：

10.1039/c7cc06523j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

FlAlyA is an endolytic enzyme with a preference for polymannuronate. The crystal structure and mutagenesis studies elucidated that the structural variations at outer uronate-binding subsites +2, +3 and -2 control the enzymatic properties of PL-7 family enzymes. Lys158 mutations changed the pH dependency and enhanced the production of mono-and disaccharides.

引用

页码：555 / 558

页数：4

共 15 条

[1]

DARVILL A, 1994, BIOCHEM SOC SYMP, P89

[2] Molecular Insight into the Role of the N-terminal Extension in the Maturation, Substrate Recognition, and Catalysis of a Bacterial Alginate Lyase from Polysaccharide Lyase Family 18 [J].

Dong, Sheng ;

Wei, Tian-Di ;

Chen, Xiu-Lan ;

Li, Chun-Yang ;

Wang, Peng ;

Xie, Bin-Bin ;

Qin, Qi-Long ;

Zhang, Xi-Ying ;

Pang, Xiu-Hua ;

Zhou, Bai-Cheng ;

Zhang, Yu-Zhong .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (43) :29558-29569

[3] Characterization of an Alginate Lyase, FlAlyA, from Flavobacterium sp Strain UMI-01 and Its Expression in Escherichia coli [J].

Inoue, Akira ;

Takadono, Kohei ;

Nishiyama, Ryuji ;

Tajima, Kenji ;

Kobayashi, Takanori ;

Ojima, Takao .

MARINE DRUGS, 2014, 12 (08) :4693-4712

[4] The carbohydrate-active enzymes database (CAZy) in 2013 [J].

Lombard, Vincent ;

Ramulu, Hemalatha Golaconda ;

Drula, Elodie ;

Coutinho, Pedro M. ;

Henrissat, Bernard .

NUCLEIC ACIDS RESEARCH, 2014, 42 (D1) :D490-D495

[5] SEQUENCE OF A GENE ENCODING A (POLY MANA) ALGINATE LYASE ACTIVE ON PSEUDOMONAS-AERUGINOSA ALGINATE [J].

MALISSARD, M ;

DUEZ, C ;

GUINAND, M ;

VACHERON, MJ ;

MICHEL, G ;

MARTY, N ;

JORIS, B ;

THAMM, I ;

GHUYSEN, JM .

FEMS MICROBIOLOGY LETTERS, 1993, 110 (01) :101-106

[6] Origin and diversity of alginate lyases of families PL-5 and-7 in Sphingomonas sp strain A1 [J].

Miyake, O ;

Ochiai, A ;

Hashimoto, W ;

Murata, K .

JOURNAL OF BACTERIOLOGY, 2004, 186 (09) :2891-2896

[7] Substrate recognition by family 7 alginate lyase from Sphingomonas sp A1 [J].

Ogura, Kohei ;

Yamasaki, Masayuki ;

Mikami, Bunzo ;

Hashimoto, Wataru ;

Murata, Kousaku .

JOURNAL OF MOLECULAR BIOLOGY, 2008, 380 (02) :373-385

[8] Crystal Structure of Family 14 Polysaccharide Lyase with pH-dependent Modes of Action [J].

Ogura, Kohei ;

Yamasaki, Masayuki ;

Yamada, Takashi ;

Mikami, Bunzo ;

Hashimoto, Wataru ;

Murata, Kousaku .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (51) :35572-35579

[9] Structure and Polymannuronate Specificity of a Eukaryotic Member of Polysaccharide Lyase Family 14 [J].

Qin, Hui-Min ;

Miyakawa, Takuya ;

Inoue, Akira ;

Nishiyama, Ryuji ;

Nakamura, Akira ;

Asano, Atsuko ;

Sawano, Yoriko ;

Ojima, Takao ;

Tanokura, Masaru .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2017, 292 (06) :2182-2190

[10] Bioethanol production from marine biomass alginate by metabolically engineered bacteria [J].

Takeda, Hiroyuki ;

Yoneyama, Fuminori ;

Kawai, Shigeyuki ;

Hashimoto, Wataru ;

Murata, Kousaku .

ENERGY & ENVIRONMENTAL SCIENCE, 2011, 4 (07) :2575-2581

← 1 2 →