Occurrence and Risk Assessment of Aflatoxin B1in Spices Marketed in Greece

The issue of food and feed contamination by fungi and aflatoxins constitutes a signficant issue for human and animal health, agriculture, and the economy. Aflatoxin B-1(AFB(1)) is considered to be the most potent liver carcinogen for humans. In this study, twenty-nine samples of spices marketed in Athens (Greece) were surveyed for microbiota and aflatoxin B(1)contamination. Four fungi genera were isolated, of whichAspergillus, Rhizopus,andFusarium spp.were the most dominant. The presence of AFB(1)was detected in 68.9% (n = 20) of the samples (mean of 9.89 ng AFB(1)g(-1)) using high-performance liquid chromatography (HPLC) with fluorescence detection. The most contaminated samples that exceeded the legislation limits were turmeric, mahalepi, garam masala, tandoori masala, with 8.73, 17.08, 28.28, and 132.70 ng AFB(1)g(-1)respectively. Moreover, 26 samples were found to be positive for bacteria contamination. In addition, a method for the determination of aflatoxin B(1)in spices was developed and validated. The recovery of the method was found to be 100.4% while the detection and the quantification limits were 0.1 ng g(-1)and 0.45 ng g(-1). Finally, a risk analysis for the consumption of the contaminated spices was assessed taking into account the maximum and the minimum measured concentrations of AFB(1).