Background free imaging of single mRNAs in live cells using split fluorescent proteins

被引：83

作者：

Wu, Bin ^{[1
,2
]}

Chen, Jiahao ^{[1
]}

Singer, Robert H. ^{[1
,2
]}

机构：

[1] Yeshiva Univ, Albert Einstein Coll Med, Dept Anat & Struct Biol, New York, NY 10033 USA

[2] Yeshiva Univ, Albert Einstein Coll Med, Gruss Lipper Biophoton Ctr, New York, NY 10033 USA

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

基金：

美国国家卫生研究院;

关键词：

LIVING CELLS; VISUALIZATION;

D O I：

10.1038/srep03615

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.

引用

页数：3

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