Ca analysis: An Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis

被引：20

作者：

Greensmith, David J. ^{[1
]}

机构：

[1] Manchester Acad Hlth Sci Ctr, Inst Cardiovasc Sci, Unit Cardiac Physiol, Core Technol Facil, Manchester M13 9NT, Lancs, England

来源：

COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE | 2014年 / 113卷 / 01期

关键词：

Intracellular calcium; Calcium transient; Fluorescent indicators; Regression analysis; Visual basic for applications; Excel; MICROSOFT EXCEL; VENTRICULAR MYOCYTES; CONFIDENCE-INTERVALS; SPREADSHEET; SIMULATION; SOLVER;

D O I：

10.1016/j.cmpb.2013.09.004

中图分类号：

TP39 [计算机的应用];

学科分类号：

081203 ; 0835 ;

摘要：

Here I present an Excel based program for the analysis of intracellular Ca transients recorded using fluorescent indicators. The program can perform all the necessary steps which convert recorded raw voltage changes into meaningful physiological information. The program performs two fundamental processes. (1) It can prepare the raw signal by several methods. (2) It can then be used to analyze the prepared data to provide information such as absolute intracellular Ca levels. Also, the rates of change of Ca can be measured using multiple, simultaneous regression analysis. I demonstrate that this program performs equally well as commercially available software, but has numerous advantages, namely creating a simplified, self-contained analysis workflow. (C) 2013 The Author. Published by Elsevier Ireland Ltd. All rights reserved.

引用

页码：241 / 250

页数：10

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