Effects of Wenxin Keli on the Action Potential and L-Type Calcium Current in Rats with Transverse Aortic Constriction-Induced Heart Failure

被引：17

作者：

Chen, Yu ^{[1
,2
]}

Li, Yang ^{[3
]}

Guo, Lili ^{[2
]}

Chen, Wen ^{[2
]}

Zhao, Mingjing ^{[4
]}

Gao, Yonghong ^{[4
]}

Wu, Aiming ^{[4
]}

Lou, Lixia ^{[4
]}

Wang, Jie ^{[2
]}

Liu, Xiaoqiu ^{[1
]}

Xing, Yanwei ^{[2
]}

机构：

[1] Shenyang Pharmaceut Univ, Shenyang 110016, Liaoning, Peoples R China

[2] China Acad Chinese Med Sci, Guanganmen Hosp, Beijing 100053, Peoples R China

[3] Chinese Peoples Liberat Army Gen Hosp, Inst Geriatr Cardiol, Beijing 100853, Peoples R China

[4] Beijing,Univ Chinese Med, Dongzhimen Hosp, Minist Educ, Key Lab Chinese Internal Med, Beijing 100700, Peoples R China

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2013年 / 2013卷

基金：

中国国家自然科学基金;

关键词：

VENTRICULAR-ARRHYTHMIAS; CARDIAC-HYPERTROPHY; CHANNEL; CA2+; REPOLARIZATION; INHIBITION; MECHANISMS; DISEASE; MODEL;

D O I：

10.1155/2013/572078

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Objective. We investigated the effects of WXKL on the action potential (AP) and the L-type calcium current (ICa-L) in normal and hypertrophied myocytes. Methods. Forty male rats were randomly divided into two groups: the control group and the transverse aortic constriction- (TAC-) induced heart failure group. Cardiac hypertrophy was induced by TAC surgery, whereas the control group underwent a sham operation. Eight weeks after surgery, single cardiac ventricular myocytes were isolated from the hearts of the rats. The APs and ICa-L were recorded using the whole-cell patch clamp technique. Results. The action potential duration (APD) of the TAC group was prolonged compared with the control group and was markedly shortened by WXKL treatment in a dose-dependent manner. The current densities of the ICa-L in the TAC group treated with 5 g/L WXKL were significantly decreased compared with the TAC group. We also determined the effect of WXKL on the gating mechanism of the ICa-L in the TAC group. We found that WXKL decreased the ICa-L by accelerating the inactivation of the channels and delaying the recovery time from inactivation. Conclusions. The results suggest that WXKL affects the AP and blocked the ICa-L, which ultimately resulted in the treatment of arrhythmias.

引用

页数：12