Crystal structure of plant pectin methylesterase

被引：111
作者：
Johansson, K
El-Ahmad, M
Friemann, R
Jörnvall, H
Markovic, O
Eklund, H [1 ]
机构：
[1] Swedish Univ Agr Sci, Dept Biol Mol, S-75124 Uppsala, Sweden
[2] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden
[3] Slovak Acad Sci, Inst Chem, Bratislava 84238, Slovakia
来源：
FEBS LETTERS | 2002年 / 514卷 / 2-3期
关键词：
crystal structure; beta-helix; pectin methylesterase; plant enzyme; catalytic mechanism; binding cleft;
D O I：
10.1016/S0014-5793(02)02372-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pectin is a principal component in the primary cell wall of plants. During cell development, pectin is modified by pectin methylesterases to give different properties to the cell wall. This report describes the first crystal structure of a plant pectin methylesterase. The P-helical structure embodies a central cleft, lined by several aromatic residues, that has been deduced to be suitable for pectin binding. The active site is found at the center of this cleft where Asp157 is suggested to act as the nucleophile, Asp136 as an acid/base and Gln113/Gln135 to form an anion hole to stabilize the transition state. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：243 / 249
页数：7
相关论文
共 50 条
  • [31] Pectin methylesterase and polygalacturonase in the developing grape skin
    Deytieux-Belleau, Christelle
    Vallet, Amelie
    Doneche, Bernard
    Geny, Laurence
    PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2008, 46 (07) : 638 - 646
  • [32] Activity of an atypical Arabidopsis thaliana pectin methylesterase
    Dedeurwaerder, Sarah
    Menu-Bouaouiche, Laurence
    Mareck, Alain
    Lerouge, Patrice
    Guerineau, Francois
    PLANTA, 2009, 229 (02) : 311 - 321
  • [33] Purification of pectin methylesterase from Orobanche aegyptiaca
    BarNun, N
    BenHod, G
    Lavi, E
    Mayer, AM
    PHYTOCHEMISTRY, 1996, 41 (02) : 403 - 406
  • [34] Development of an immunological toolbox to detect endogenous and exogenous pectin methylesterase in plant-based food products
    Vandevenne, Evelien
    Van Buggenhout, Sandy
    Peeters, Miet
    Compernolle, Griet
    Declerck, Paul J.
    Hendrickx, Marc E.
    Van Loey, Ann
    Gils, Ann
    FOOD RESEARCH INTERNATIONAL, 2011, 44 (04) : 931 - 939
  • [35] Plant pectin methylesterase and its inhibitor from kiwi fruit: Interaction analysis by surface plasmon resonance
    Jolie, Ruben P.
    Duvetter, Thomas
    Houben, Ken
    Vandevenne, Evelien
    Van Loey, Ann M.
    Declerck, Paul J.
    Hendrickx, Marc E.
    Gils, Ann
    FOOD CHEMISTRY, 2010, 121 (01) : 207 - 214
  • [36] Pectin methylesterase inhibitor cDNA from kiwi fruit
    Kohei Irifune
    Tetsuya Nishida
    Hiroko Egawa
    Aya Nagatani
    Plant Cell Reports, 2004, 23 : 333 - 338
  • [37] Role of the leader sequence in tobacco pectin methylesterase secretion
    Dorokhov, Yuri L.
    Skurat, Eugene V.
    Frolova, Olga Yu.
    Gasanova, Tatjana V.
    Ivanov, Peter A.
    Ravin, Nikolay V.
    Skryabin, Konstantin G.
    Makinen, Kristiina M.
    Klimyuk, Viktor I.
    Gleba, Yuri Yu.
    Atabekov, Joseph G.
    FEBS LETTERS, 2006, 580 (13) : 3329 - 3334
  • [38] Pectin methylesterase pattern in flax seedlings during their development
    Alexandre, F
    Morvan, O
    Gaffe, J
    Mareck, A
    Jauneau, A
    Dauchel, H
    Balange, AP
    Morvan, C
    PLANT PHYSIOLOGY AND BIOCHEMISTRY, 1997, 35 (06) : 427 - 436
  • [39] Pectin methylesterase inhibitor cDNA from kiwi fruit
    Irifune, K
    Nishida, T
    Egawa, H
    Nagatani, A
    PLANT CELL REPORTS, 2004, 23 (05) : 333 - 338
  • [40] Acerola's pectin methylesterase: studies of heat inactivation
    de Assis, SA
    Lima, DC
    Oliveira, OMMD
    FOOD CHEMISTRY, 2000, 71 (04) : 465 - 467
12345