Two-photon probes based on arylsulfonyl azides: Fluorescence detection and imaging of biothiols

被引：22

作者：

Singha, Subhankar ^{[1
,2
]}

Kim, Dokyoung ^{[1
,2
]}

Rao, Alla Sreenivasa ^{[1
,2
]}

Wang, Taejun ^{[3
,4
]}

Kim, Ki Hean ^{[3
,4
]}

Lee, Kyung-Ha ^{[5
]}

Kim, Kyong-Tai ^{[5
]}

Ahn, Kyo Han ^{[1
,2
]}

机构：

[1] POSTECH, Dept Chem, Pohang 790784, South Korea

[2] POSTECH, Ctr Electrophoto Behav Adv Mol Syst, Pohang 790784, South Korea

[3] POSTECH, Dept Mech Engn, Pohang 790784, South Korea

[4] POSTECH, Div Integrat Biosci & Biotechnol, Pohang 790784, South Korea

[5] POSTECH, Dept Life Sci, Pohang 790784, South Korea

来源：

DYES AND PIGMENTS | 2013年 / 99卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Biothiols; Fluorescent probes; Two-photon imaging; Arylsulfonyl azides; Arylsulfonamide; Mitochondrial thiols; HYDROGEN-SULFIDE; ELECTRON-TRANSFER; CYSTEINE; GLUTATHIONE; THIOLS; QUANTIFICATION; HOMOCYSTEINE; SENSOR; ASSAY;

D O I：

10.1016/j.dyepig.2013.05.008

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Two-photon probes for biothiols are developed based on arylsulfonyl azides for the first time. Thus, 6-(dimethylamino)naphthalene-2-sulfonyi azide and its triphenylphosphonium derivative undergo a fast reduction with turn-on fluorescence change by typical biothiols such as hydrogen sulfide, glutathione, cysteine, and homocysteine to produce the corresponding sulfonamides that is two-photon excitable. The cationic probe enables the selective imaging of the biothiols in mitochondria by two-photon fluorescence microscopy. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：308 / 315

页数：8

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