Cathepsin B-like proteinase is involved in the decomposition of the adult fat body of Helicoverpa armigera

被引:35
作者
Yang, XM [1 ]
Hou, LJ [1 ]
Dong, DJ [1 ]
Shao, HL [1 ]
Wang, JX [1 ]
Zhao, XF [1 ]
机构
[1] Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China
关键词
Helicoverpa armigera; cathepsin B-like proteinase; decomposition of fat body; programmed cell death; metamorphosis;
D O I
10.1002/arch.20115
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cathepsin B-like pioteinase (HCB, EC 3.4.22.1) is expressed in Helicoverpa armigera oocytes and adult fat bodies. Previous work has revealed that HCB plays a key role in the degradation of yolk proteins during embryogenesis. This study investigated the function and regulatory activation of HCB in adult fat bodies during aging and oogenesis. The HCB transcript was detected at all stages from larval to adult fat bodies with Northern blot analysis. Pro-HCB was also detected in fat bodies at these stages with an immunoblot assay using a monoclonal antibody against HCB. However, mature HCB and its activity were only detected in fat bodies of pre-adults and adults. This evidence suggested that HCB is regulated post-translationally by activation of the pro-enzyme during the pupa-adult metamorphosis. The activation of HCB was coupled with the expression of hormone receptor 3 (HHR3), and was up-regulated by the ecdysteroid agonist, RH-2485, suggesting that HCB activation is related to the ecdysone regulatory system. The decomposition of the adult fat bodies during aging and cogenesis was found to occur via programmed cell death, in which HCB took part.
引用
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页码:1 / 10
页数:10
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