Electrochemically controlled solid-phase micro-extraction of proline using a nanostructured film of polypyrrole, and its determination by ion mobility spectrometry

被引：19

作者：

Kalhor, Hamideh ^{[1
]}

Ameli, Akram ^{[1
]}

Alizadeh, Naader ^{[1
]}

机构：

[1] Tarbiat Modares Univ, Dept Chem, Fac Basic Sci, Tehran, Iran

来源：

MICROCHIMICA ACTA | 2013年 / 180卷 / 9-10期

基金：

美国国家科学基金会;

关键词：

Proline; EC-SPME; Nanostructural conducting polypyrrole; Ion mobility spectrometry; TANDEM MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; MOLECULARLY IMPRINTED POLYMER; FREE AMINO-ACIDS; PROSTATE-CANCER; GAS-CHROMATOGRAPHY; HUMAN URINE; ANTIGEN PSA; MICROEXTRACTION; SAMPLES;

D O I：

10.1007/s00604-013-0984-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report on a fast, simple and accurate method for the determination of proline in urine samples by employing a nanostructured film of conducting polypyrrole for electrochemically controlled solid-phase microextraction, and ion mobility spectrometry (IMS) for detection. This method has the advantages of simple sample preparation and a sensitivity of IMS to proline that is higher than that for other amino acids. The calibration curve is linear in the range of 0.5-60 mu g L-1 (4-521 nmol L-1), and the detection limit is 0.2 mu g L-1. The electrochemical potentials for uptake and release were optimized. The method was successfully applied to the clean-up and quantitation of trace amounts of proline in urine samples.

引用

页码：783 / 789

页数：7

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