Study on Determination of Glucose in Amylofermentation Liquid Using Ultraviolet Spectrophotometry

被引:2
作者
He, Baoshan [1 ]
Gao, Na [1 ]
Wei, Fang [1 ]
Lu, Qiyu [1 ]
机构
[1] Henan Univ Technol, Sch Food Sci & Technol, Zhengzhou 450001, Henan, Peoples R China
来源
MATERIALS PROCESSING TECHNOLOGY II, PTS 1-4 | 2012年 / 538-541卷
关键词
Glucose; Ultraviolet spectrophotometry; Potassium titanyl oxalate; Glucose oxidase; SENSOR; BIOSENSOR;
D O I
10.4028/www.scientific.net/AMR.538-541.2434
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
In the presence of glucose oxidase, glucose in samples was oxygenated to hydrogen peroxide, the solution turned from colourless to yellow upon the reaction of potassium titanyl oxalate to the generated hydrogen peroxide. Using ultraviolet spectrophotometry, a new optical method for detecting glucose in amylofermentation liquid has been established. Results demonstrated that glucose concentrations were proportional to absorbance at the maximum absorption wavelength of 380 nm. A favorable linearity was presented in the range of 1 mmol/L to 60 mmol/L. The linear coeffciency was 0.993. This method was simple, reliable, and could be used for determing glucose in samples.
引用
收藏
页码:2434 / 2437
页数:4
相关论文
共 7 条
[1]   A colorimetric titration method for quantification of millimolar glucose in a pH 7.4 aqueous phosphate buffer [J].
Boduroglu, S ;
El Khoury, JM ;
Reddy, DV ;
Rinaldi, PL ;
Hu, J .
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2005, 15 (17) :3974-3977
[2]   A viscosity-dependent affinity sensor for continuous monitoring of glucose in biological fluids [J].
Boss, Christophe ;
Meurville, Eric ;
Sallese, Jean-Michel ;
Ryser, Peter .
BIOSENSORS & BIOELECTRONICS, 2011, 30 (01) :223-228
[3]   OPTIMIZATION OF A BIOSENSOR FOR INSITU FERMENTATION MONITORING OF GLUCOSE-CONCENTRATION [J].
BRADLEY, J ;
SCHMID, RD .
BIOSENSORS & BIOELECTRONICS, 1991, 6 (08) :669-674
[4]   Nonenzymatic glucose sensor based on CuO microfibers composed of CuO nanoparticles [J].
Cao, Fei ;
Gong, Jian .
ANALYTICA CHIMICA ACTA, 2012, 723 :39-44
[5]   Direct electrochemistry of glucose oxidase immobilized on nanostructured gold thin films and its application to bioelectrochemical glucose sensor [J].
Qiu, Cuicui ;
Wang, Xia ;
Liu, Xueying ;
Hou, Shifeng ;
Ma, Houyi .
ELECTROCHIMICA ACTA, 2012, 67 :140-146
[6]   Continuous glucose sensor using novel genetically engineered binding polypeptides towards in vivo applications [J].
Siegrist, Jonathan ;
Kazarian, Troy ;
Ensor, Charles ;
Joel, Smita ;
Madou, Marc ;
Wang, Ping ;
Daunert, Sylvia .
SENSORS AND ACTUATORS B-CHEMICAL, 2010, 149 (01) :51-58
[7]   Monitoring of the glucose concentration during microbial fermentation using a novel mass-producible biosensor suitable for on-line use [J].
Tothill, IE ;
Newman, JD ;
White, SF ;
Turner, APF .
ENZYME AND MICROBIAL TECHNOLOGY, 1997, 20 (08) :590-596