共 7 条
Study on Determination of Glucose in Amylofermentation Liquid Using Ultraviolet Spectrophotometry
被引:2
作者:
He, Baoshan
[1
]
Gao, Na
[1
]
Wei, Fang
[1
]
Lu, Qiyu
[1
]
机构:
[1] Henan Univ Technol, Sch Food Sci & Technol, Zhengzhou 450001, Henan, Peoples R China
来源:
MATERIALS PROCESSING TECHNOLOGY II, PTS 1-4
|
2012年
/
538-541卷
关键词:
Glucose;
Ultraviolet spectrophotometry;
Potassium titanyl oxalate;
Glucose oxidase;
SENSOR;
BIOSENSOR;
D O I:
10.4028/www.scientific.net/AMR.538-541.2434
中图分类号:
T [工业技术];
学科分类号:
08 ;
摘要:
In the presence of glucose oxidase, glucose in samples was oxygenated to hydrogen peroxide, the solution turned from colourless to yellow upon the reaction of potassium titanyl oxalate to the generated hydrogen peroxide. Using ultraviolet spectrophotometry, a new optical method for detecting glucose in amylofermentation liquid has been established. Results demonstrated that glucose concentrations were proportional to absorbance at the maximum absorption wavelength of 380 nm. A favorable linearity was presented in the range of 1 mmol/L to 60 mmol/L. The linear coeffciency was 0.993. This method was simple, reliable, and could be used for determing glucose in samples.
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页码:2434 / 2437
页数:4
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