Visualizing viral assemblies in a nanoscale biosphere

被引：32

作者：

Gilmore, Brian L. ^{[1
]}

Showalter, Shannon P. ^{[1
]}

Dukes, Madeline J. ^{[2
]}

Tanner, Justin R. ^{[1
]}

Demmert, Andrew C. ^{[1
]}

McDonald, Sarah M. ^{[1
]}

Kelly, Deborah F. ^{[1
]}

机构：

[1] Virginia Tech, Carilion Res Inst, Roanoke, VA 24016 USA

[2] Protochips Inc, Applicat Sci, Raleigh, NC 27607 USA

来源：

LAB ON A CHIP | 2013年 / 13卷 / 02期

关键词：

NEAR-ATOMIC RESOLUTION; ELECTRON-MICROSCOPY; PURIFICATION; GRAPHENE; GROWTH; EM;

D O I：

10.1039/c2lc41008g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a novel microfluidic platform to examine biological assemblies at high-resolution. We have engineered a functionalized chamber that serves as a "nanoscale biosphere'' to capture and maintain rotavirus double-layered particles (DLPs) in a liquid environment. The chamber can be inserted into the column of a transmission electron microscope while being completely isolated from the vacuum system. This configuration allowed us to determine the structure of biological complexes at nanometer-resolution within a self-contained vessel. Images of DLPs were used to calculate the first 3D view of macromolecules in solution. We refer to this new fluidic visualization technology as in situ molecular microscopy.

引用

页码：216 / 219

页数：4

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