A physical sciences network characterization of non-tumorigenic and metastatic cells

被引：128

作者：

Agus, David B. ^{[1
]}

Alexander, Jenolyn F. ^{[2
]}

Arap, Wadih ^{[3
]}

Ashili, Shashanka ^{[4
]}

Aslan, Joseph E. ^{[5
,6
]}

Austin, Robert H. ^{[7
]}

Backman, Vadim ^{[8
]}

Bethel, Kelly J. ^{[9
]}

Bonneau, Richard ^{[10
]}

Chen, Wei-Chiang ^{[11
]}

Chen-Tanyolac, Chira ^{[12
,13
]}

Choi, Nathan C. ^{[1
]}

Curley, Steven A. ^{[14
]}

Dallas, Matthew ^{[11
]}

Damania, Dhwanil ^{[8
]}

Davies, Paul C. W. ^{[15
]}

Decuzzi, Paolo ^{[2
]}

Dickinson, Laura ^{[11
]}

Estevez-Salmeron, Luis ^{[12
,13
]}

Estrella, Veronica ^{[16
,17
]}

Ferrari, Mauro ^{[2
]}

Fischbach, Claudia ^{[18
]}

Foo, Jasmine ^{[19
]}

Fraley, Stephanie I. ^{[11
]}

Frantz, Christian ^{[20
,21
]}

Fuhrmann, Alexander ^{[22
]}

Gascard, Philippe ^{[12
,13
]}

Gatenby, Robert A. ^{[16
,17
]}

Geng, Yue ^{[18
]}

Gerecht, Sharon ^{[11
]}

Gillies, Robert J. ^{[16
,17
]}

Godin, Biana ^{[2
]}

Grady, William M. ^{[23
,24
]}

Greenfield, Alex ^{[10
]}

Hemphill, Courtney ^{[4
]}

Hempstead, Barbara L. ^{[25
]}

Hielscher, Abigail ^{[11
]}

Hillis, W. Daniel ^{[1
,26
]}

Holland, Eric C. ^{[27
]}

Ibrahim-Hashim, Arig ^{[16
,17
]}

Jacks, Tyler ^{[28
,29
,30
]}

Johnson, Roger H. ^{[4
]}

Joo, Ahyoung ^{[1
]}

Katz, Jonathan E. ^{[1
]}

Kelbauskas, Laimonas ^{[4
]}

Kesselman, Carl ^{[31
]}

King, Michael R. ^{[18
]}

Konstantopoulos, Konstantinos ^{[11
]}

Kraning-Rush, Casey M. ^{[18
]}

Kuhn, Peter ^{[32
]}

机构：

[1] Univ So Calif, Keck Sch Med, Ctr Appl Mol Med, Los Angeles, CA 90033 USA

[2] Methodist Hosp, Dept Nanomed, Res Inst, Houston, TX 77030 USA

[3] Univ Texas MD Anderson Canc Ctr, David H Koch Ctr, Houston, TX 77030 USA

[4] Arizona State Univ, Ctr Biosignatures Discovery Automat, Biodesign Inst, Tempe, AZ 85287 USA

[5] Oregon Hlth & Sci Univ, Dept Biomed Engn, Sch Med, Portland, OR 97239 USA

[6] Oregon Hlth & Sci Univ, Dept Cell & Dev Biol, Sch Med, Portland, OR 97239 USA

[7] Princeton Univ, Dept Phys, Princeton, NJ 08544 USA

[8] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA

[9] Scripps Clin, Dept Pathol, La Jolla, CA 92037 USA

[10] NYU, Dept Biol, Dept Comp Sci, New York, NY 10003 USA

[11] Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD 21218 USA

[12] Univ Calif San Francisco, Dept Pathol, San Francisco, CA 94143 USA

[13] Univ Calif San Francisco, UCSF Helen Diller Family Comprehens Canc Ctr, San Francisco, CA 94143 USA

[14] Univ Texas MD Anderson Canc Ctr, Dept Surg Oncol, Houston, TX 77030 USA

[15] Arizona State Univ, Beyond Ctr Fundamental Concepts Sci, Tempe, AZ 85287 USA

[16] H Lee Moffitt Canc Ctr & Res Inst, Dept Radiol, Tampa, FL 33612 USA

[17] H Lee Moffitt Canc Ctr & Res Inst, Dept Integrated Math Oncol, Tampa, FL 33612 USA

[18] Cornell Univ, Dept Biomed Engn, Ithaca, NY 14853 USA

[19] Univ Minnesota Twin Cities, Sch Math, Minneapolis, MN 55455 USA

[20] Univ Calif San Francisco, Dept Surg, San Francisco, CA 94143 USA

[21] Univ Calif San Francisco, Ctr Bioengn & Tissue Regenerat, San Francisco, CA 94143 USA

[22] Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA

[23] Fred Hutchinson Canc Res Ctr, Div Clin Res, Seattle, WA 98109 USA

[24] Univ Washington, Dept Med, Seattle, WA 98195 USA

[25] Weill Cornell Med Coll, Dept Med, New York, NY 10065 USA

[26] Appl Minds Inc, Glendale, CA 91201 USA

[27] Mem Sloan Kettering Canc Ctr, Canc Biol & Genet Program, Dept Neurosurg, New York, NY 10065 USA

[28] MIT, Dept Biol, Cambridge, MA 02139 USA

[29] MIT, Koch Inst Integrat Canc Res, Cambridge, MA 02139 USA

[30] MIT, Howard Hughes Med Inst, Cambridge, MA 02139 USA

[31] Univ So Calif, Inst Informat Sci, Marina Del Rey, CA 90292 USA

[32] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[33] MIT, Dept Phys, Cambridge, MA 02139 USA

[34] Northwestern Univ, Div Hematol Oncol, Robert H Lurie Comprehens Canc Ctr, Feinberg Sch Med, Chicago, IL 60611 USA

[35] Univ Calif Berkeley, Dept Phys, Biophys Grad Grp, Berkeley, CA 94720 USA

[36] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

[37] H Lee Moffitt Canc Ctr & Res Inst, Dept Analyt Microscopy, Tampa, FL 33612 USA

[38] Stanford Univ, Dept Radiol, Sch Med, Stanford, CA 94305 USA

[39] Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA

[40] Harvard Univ, Sch Publ Hlth, Dept Biostat & Computat Biol, Dana Farber Canc Inst, Boston, MA 02115 USA

[41] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA

[42] Northwestern Univ, Dept Chem, Chem Life Proc Inst, Evanston, IL 60208 USA

[43] Johns Hopkins Univ, Sch Med, Vasc Program, Inst Cell Engn, Baltimore, MD 21205 USA

[44] Johns Hopkins Univ, Sch Med, McKusick Nathans Inst Genet Med, Baltimore, MD 21205 USA

[45] Johns Hopkins Univ, Sch Med, Dept Pediat, Baltimore, MD 21205 USA

[46] Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA

[47] Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21205 USA

[48] Johns Hopkins Univ, Sch Med, Dept Radiat Oncol, Baltimore, MD 21205 USA

[49] Johns Hopkins Univ, Sch Med, Dept Biol Chem, Baltimore, MD 21205 USA

[50] Cornell Univ, Sch Chem & Biomol Engn, Ithaca, NY 14853 USA

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

关键词：

LYSYL OXIDASE; CANCER; MIGRATION; MORPHOLOGY; MECHANISM; SURVIVAL; ADHESION; PROTEIN; LINES;

D O I：

10.1038/srep01449

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.

引用

页数：12

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