Identification of an O-linked repetitive glycan chain of the polar flagellum flagellin of Azospirillum brasilense Sp7

被引：22

作者：

Belyakov, Alexei Ye. ^{[1
]}

Burygin, Gennady L. ^{[1
]}

Arbatsky, Nikolai P. ^{[2
]}

Shashkov, Alexander S. ^{[2
]}

Selivanov, Nikolai Yu. ^{[1
]}

Matora, Larisa Yu. ^{[1
]}

Knirel, Yuriy A. ^{[2
]}

Shchyogolev, Sergei Yu. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410049, Russia

[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia

来源：

CARBOHYDRATE RESEARCH | 2012年 / 361卷

关键词：

O-linked polysaccharide; Polar flagellum flagellin; Azospirillum brasilense; PSEUDOMONAS-AERUGINOSA; GLYCOSYLATION; MOTILITY;

D O I：

10.1016/j.carres.2012.08.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This is the first report to have identified an O-linked repetitive glycan in bacterial flagellin, a structural protein of the flagellum. Studies by sugar analysis, Smith degradation, H-1 and C-13 NMR spectroscopy, and mass spectrometry showed that the glycan chains of the polar flagellum flagellin of the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp7 are represented by a polysaccharide with a molecular mass of 7.7 kDa, which has a branched tetrasaccharide repeating unit of the following structure: -> 3)-alpha-L-Rhap-(1 -> 3)-beta-D-Galp-(1 -> 3)-beta-D-GlcpNAc-(1 -> 4 up arrow 1 alpha-L-Fucp (C) 2012 Published by Elsevier Ltd.

引用

页码：127 / 132

页数：6

共 26 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] Detection of a sheath on Azospirillum brasilense polar flagellum [J].

Burygin, G. L. ;

Shirokov, A. A. ;

Shelud'ko, A. V. ;

Katsy, E. I. ;

Shchygolev, S. Yu. ;

Matora, L. Yu. .

MICROBIOLOGY, 2007, 76 (06) :728-734

[3]

CARLSON DM, 1966, J BIOL CHEM, V241, P2984

[4] IDENTIFICATION AND MAPPING OF LOCI INVOLVED IN MOTILITY, ADSORPTION TO WHEAT ROOTS, COLONY MORPHOLOGY, AND GROWTH IN MINIMAL MEDIUM ON THE AZOSPIRILLUM-BRASILENSE SP7 90-MDA PLASMID [J].

CROES, C ;

VANBASTELAERE, E ;

DECLERCQ, E ;

EYERS, M ;

VANDERLEYDEN, J ;

MICHIELS, K .

PLASMID, 1991, 26 (02) :83-93

[5]

Dobereiner J., 1976, Proceedings of the 1st International Symposium on Nitrogen Fixation [Newton, W.E.

[6]

Nyman, C.J. Editors]., P518

[7] Characterization of a post-translational modification of Campylobacter flagellin: Identification of a sero-specific glycosyl moiety [J].

Doig, P ;

Kinsella, N ;

Guerry, P ;

Trust, TJ .

MOLECULAR MICROBIOLOGY, 1996, 19 (02) :379-387

[8] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].

DUBOIS, M ;

GILLES, K ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

NATURE, 1951, 168 (4265) :167-167

[9] Glycosylation Regulates Specific Induction of Rice Immune Responses by Acidovorax avenae Flagellin [J].

Hirai, Hiroyuki ;

Takai, Ryota ;

Iwano, Megumi ;

Nakai, Masaru ;

Kondo, Machiko ;

Takayama, Seiji ;

Isogai, Akira ;

Che, Fang-Sik .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (29) :25519-25530

[10]

Katzy E. I., 2003, MOL GENETIC PROCESSE

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