Pattern of activation of human antigen presenting cells by genotype GII.4 norovirus virus-like particles

被引：12
|
作者：
Ponterio, Eleonora [1 ]
Petrizzo, Annacarmen [2 ]
Di Bartolo, Ilaria [1 ]
Buonaguro, Franco Maria [2 ]
Buonaguro, Luigi [2 ]
Ruggeri, Franco Maria [1 ]
机构：
[1] Ist Super Sanita, Dept Vet Publ Hlth & Food Safety, I-00161 Rome, Italy
[2] Ist Nazl Studio & Cura Tumori Fdn Pascale IRCCS, Dept Expt Oncol, Lab Mol Biol & Viral Oncol, I-80131 Naples, Italy
来源：
JOURNAL OF TRANSLATIONAL MEDICINE | 2013年 / 11卷
关键词：
Norovirus; Immunology; PBMCs; VLPs; GENE-EXPRESSION PROFILE; DENDRITIC CELLS; MONOCLONAL-ANTIBODIES; IMMUNE-RESPONSES; VACCINE; MUCOSAL; IMMUNOGENICITY; IMMUNIZATION; INFECTION; CYTOKINE;
D O I：
10.1186/1479-5876-11-127
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background: Virus-like particles (VLPs) from an Italian GII.4 norovirus strain were used to investigate activation and maturation of circulating antigen presenting cells (APCs) of human origin. Methods: Peripheral blood mononuclear cells (PBMCs) isolated from five healthy subjects were pulsed ex vivo with VLPs, and stained with a set of monoclonal antibodies (MAbs) for phenotypic analysis by flow cytometry. Cytokine release in cell supernatants was investigated by ELISA. Results: Norovirus VLPs induced activation and maturation of circulating APCs derived from the five donors, as well as production of IL-6, IFN-gamma and TNF-alpha cytokines. Conclusions: The present results suggest that VLPs can activate antigen presenting cells for an efficient induction of the adaptive immune response.
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页数：7
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