Pattern of activation of human antigen presenting cells by genotype GII.4 norovirus virus-like particles

被引：12
|
作者：
Ponterio, Eleonora [1 ]
Petrizzo, Annacarmen [2 ]
Di Bartolo, Ilaria [1 ]
Buonaguro, Franco Maria [2 ]
Buonaguro, Luigi [2 ]
Ruggeri, Franco Maria [1 ]
机构：
[1] Ist Super Sanita, Dept Vet Publ Hlth & Food Safety, I-00161 Rome, Italy
[2] Ist Nazl Studio & Cura Tumori Fdn Pascale IRCCS, Dept Expt Oncol, Lab Mol Biol & Viral Oncol, I-80131 Naples, Italy
来源：
JOURNAL OF TRANSLATIONAL MEDICINE | 2013年 / 11卷
关键词：
Norovirus; Immunology; PBMCs; VLPs; GENE-EXPRESSION PROFILE; DENDRITIC CELLS; MONOCLONAL-ANTIBODIES; IMMUNE-RESPONSES; VACCINE; MUCOSAL; IMMUNOGENICITY; IMMUNIZATION; INFECTION; CYTOKINE;
D O I：
10.1186/1479-5876-11-127
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background: Virus-like particles (VLPs) from an Italian GII.4 norovirus strain were used to investigate activation and maturation of circulating antigen presenting cells (APCs) of human origin. Methods: Peripheral blood mononuclear cells (PBMCs) isolated from five healthy subjects were pulsed ex vivo with VLPs, and stained with a set of monoclonal antibodies (MAbs) for phenotypic analysis by flow cytometry. Cytokine release in cell supernatants was investigated by ELISA. Results: Norovirus VLPs induced activation and maturation of circulating APCs derived from the five donors, as well as production of IL-6, IFN-gamma and TNF-alpha cytokines. Conclusions: The present results suggest that VLPs can activate antigen presenting cells for an efficient induction of the adaptive immune response.
引用
收藏
页数：7
相关论文
共 50 条
  • [31] Development of Virus-Like Particles (VLPs) for Hepatitis C Virus genotype 4: a novel approach for vaccine development in Egypt
    Ali, Ahmed A.
    Azouz, Rasha A. M.
    Hussein, Nahla A.
    El-Shenawy, Reem
    Helmy, Naiera M.
    El-Abd, Yasmine S.
    Tabll, Ashraf A.
    BMC BIOTECHNOLOGY, 2025, 25 (01)
  • [32] Bacterial Surface-Displayed GII.4 Human Norovirus Capsid Proteins Bound to HBGA-Like Molecules in Romaine Lettuce
    Wang, Ming
    Rong, Shaofeng
    Tian, Peng
    Zhou, Yue
    Guan, Shimin
    Li, Qianqian
    Wang, Dapeng
    FRONTIERS IN MICROBIOLOGY, 2017, 8
  • [33] Physical interaction of human papillomavirus virus-like particles with immune cells
    Da Silva, DM
    Velders, MP
    Nieland, JD
    Schiller, JT
    Nickoloff, BJ
    Kast, WM
    INTERNATIONAL IMMUNOLOGY, 2001, 13 (05) : 633 - 641
  • [34] Disulfide stabilization of human norovirus GI.1 virus-like particles focuses immune response toward blockade epitopes
    Verardi, Raffaello
    Lindesmith, Lisa C.
    Tsybovsky, Yaroslav
    Gorman, Jason
    Chuang, Gwo-Yu
    Edwards, Caitlin E.
    Brewer-Jensen, Paul D.
    Mallory, Michael L.
    Ou, Li
    Schon, Arne
    Shi, Wei
    Tully, Ena S.
    Georgiou, George
    Baric, Ralph S.
    Kwong, Peter D.
    NPJ VACCINES, 2020, 5 (01)
  • [35] Enterovirus-71 Virus-Like Particles Induce the Activation and Maturation of Human Monocyte-Derived Dendritic Cells through TLR4 Signaling
    Lin, Yu-Li
    Hu, Yu-Chen
    Liang, Cheng-Chao
    Lin, Shih-Yeh
    Liang, Yu-Chih
    Yuan, Hui-Ping
    Chiang, Bor-Luen
    PLOS ONE, 2014, 9 (10):
  • [36] Antigenicity study of the yeast-generated human parvovirus 4 (PARV4) virus-like particles
    Lazutka, Justas
    Simutis, Karolis
    Matulis, Paulius
    Petraityte-Burneikiene, Rasa
    Kucinskaite-Kodze, Indre
    Simanavicius, Martynas
    Tamosiunas, Paulius Lukas
    VIRUS RESEARCH, 2021, 292
  • [37] Topically applied virus-like particles containing HIV-1 Pr55gag protein reach skin antigen-presenting cells after mild skin barrier disruption
    Rancan, Fiorenza
    Afraz, Zahra
    Hadam, Sabrina
    Weiss, Lina
    Perrin, Helene
    Kliche, Alexander
    Schrade, Petra
    Bachmann, Sebastian
    Schaefer-Korting, Monika
    Blume-Peytavi, Ulrike
    Wagner, Ralf
    Combadiere, Behazine
    Vogt, Annika
    JOURNAL OF CONTROLLED RELEASE, 2017, 268 : 296 - 304
  • [38] Expression and characterization of human group C rotavirus virus-like particles in insect cells
    Clark, Kristina B.
    Lin, Seh-Ching
    Humphrey, Charles
    Foytich, Kimberly
    Esona, Mathew
    Wang, Yuhuan
    Liu, Merry
    Jiang, Baoming
    VIROLOGY, 2009, 387 (02) : 267 - 272
  • [39] Japanese encephalitis genotype I virus-like particles stably expressed in BHK-21 cells serves as potential antigen in JE IgM ELISA
    Mali, D. N.
    Bondre, V. P.
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2022, 106 (5-6) : 1945 - 1955
  • [40] Macropinocytosis Is the Principal Uptake Mechanism of Antigen-Presenting Cells for Allergen-Specific Virus-like Nanoparticles
    Kraus, Armin
    Kratzer, Bernhard
    Sehgal, Al Nasar Ahmed
    Trapin, Doris
    Khan, Matarr
    Boucheron, Nicole
    Pickl, Winfried F.
    VACCINES, 2024, 12 (07)
12345