Differential pulse voltammetric enzyme-linked immunoassay for the determination of Helicobacter pylori specific immunoglobulin G (IgG) antibody

被引：51

作者：

He, YN

Chen, HY

Zheng, JJ

Zhang, GY

Chen, ZL

机构：

[1] NANJING UNIV,INST COORDINAT CHEM,DEPT CHEM,NANJING 210093,PEOPLES R CHINA

[2] NANJING CHILDRENS HOSP,NANJING 210008,PEOPLES R CHINA

来源：

TALANTA | 1997年 / 44卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Helicobacter pylori; immunoglobulin G (IgG) antibody; serum;

D O I：

10.1016/S0039-9140(96)02120-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A differential pulse voltammetric enzyme-linked immunoassay for the determination of helicobacter pylori (H. pylori) specific IgG antibody in human serum has been developed. The method is based on coupling the oxidation reaction of 3,3',5,5'-tetramethylbenzidine (TMB)-H2O2 that is catalysed by horseradish peroxidase-IgG(HRP-IgG) conjugate with the electro-reduction of the enzymatic product to measure the activity of HRP-IgG. The latter reaction exhibits a sensitive differential pulse voltammetric response at 0.1 V (versus Ag/AgCl) in pH 4.0 acetate buffer solution. So, the H. pylori specific IgG antibody could be detected. The detection limit of present method for H. pylori specific IgG antibody was 1.0 units ml(-1), which was about seven times lower than that obtained by traditional spectrophotometric ELISA procedure. (C) 1997 Elsevier Science B.V.

引用

页码：823 / 830

页数：8

共 10 条

[1] AVIDIN-BIOTIN BASED ELECTROCHEMICAL IMMUNOASSAY FOR THYROTROPIN [J].