Immulectin-4 from the tobacco hornworm Manduca sexta binds to lipopolysaccharide and lipoteichoic acid

被引：108

作者：

Yu, XQ ^{[1
]}

Ling, E

Tracy, ME

Zhu, Y

机构：

[1] Univ Missouri, Sch Biol Sci, Div Cell Biol & Biophys, Kansas City, MO 64110 USA

[2] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA

来源：

INSECT MOLECULAR BIOLOGY | 2006年 / 15卷 / 02期

关键词：

C-type lectin; immulectin; lipopolysaccharide; encapsulation; melanization;

D O I：

10.1111/j.1365-2583.2006.00618.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insect C-type lectins function as pattern recognition receptors in innate immunity. In the tobacco hornworm Manduca sexta, we have previously isolated three C-type lectins named immulectins, which are involved in innate immune responses. Here, we report a new member of the immulectin family, immulectin-4 (IML-4). IML-4 mRNA was detected in the fat body of control larvae and was induced in the fat body when larvae were injected with bacteria. Recombinant IML-4 bound to bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and the binding activity was not affected by addition of calcium or EGTA. IML-4 agglutinated bacteria and yeast, and agglutination of Escherichia coli by IML-4 was concentration- and calcium-dependent. IML-4 also enhanced haemocyte encapsulation and melanization.

引用

页码：119 / 128

页数：10

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