Resistance to apoptosis of harringtonine-resistant HL60 cells induced by tetrandrine

被引：0

作者：

He, QY ^{[1
]}

Zhang, HQ ^{[1
]}

Pang, DB ^{[1
]}

Chi, XS ^{[1
]}

Xue, SB ^{[1
]}

机构：

[1] CHINESE ACAD TRADIT CHINESE MED, INST BASIC THEORY TCM, BEIJING 100700, PEOPLES R CHINA

来源：

ACTA PHARMACOLOGICA SINICA | 1996年 / 17卷 / 06期

关键词：

apoptosis; tetrandrine; harringtonines; leukemia HL60; c-myc genes; drug resistance; phosphorylation;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AIM: To study the mechanism of resistance to, apoptosis in the harringtonine (Har)-resistant HL60 cells with tetrandrine (Tet). METHODS: Growth inhibition, flow cytometry, DNA agarose gel electrophoresis, protein phosphorylation, and RNA dot hybridization. RESULTS: The resistant cells had no cross resistance to Tet. Tet induced the sensitive but not the Har-resistant HL60 cells to apoptosis. The high phosphorylation of protein <30 kDa occurred when the resistant cells were treated with Tet. Tet and Har increased the expression of c-myc mRNA in the sensitive HL60 cells. The expression of c-myc mRNA in the resistant cells was obviously decreased and almost not changed in treatment with Tet and Har. CONCLUSION: Tet induced the sensitive but not the Har-resistant HL60 cells to apoptosis, and the resistance to apoptosis induced by Tet was associated with the high protein phosphorylation and reduction of the expression of c-myc mRNA.

引用

页码：545 / 549

页数：5

共 12 条

[1] BAXTER GD, 1992, J IMMUNOL, V148, P1949
[2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION
CHOMCZYNSKI, P
SACCHI, N
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) : 156 - 159
[4] INDUCTION OF APOPTOSIS IN FIBROBLASTS BY C-MYC PROTEIN
EVAN, GI
WYLLIE, AH
GILBERT, CS
LITTLEWOOD, TD
LAND, H
BROOKS, M
WATERS, CM
PENN, LZ
HANCOCK, DC
[J]. CELL, 1992, 69 (01) : 119 - 128
[5] FANG M, 1995, CHINESE SCI BULL, V40, P939
[6] Fang M., 1994, Yaoxue Xuebao, V29, P891
[7] He QY, 1996, ACTA PHARM SINIC, V17, P179
[8] HE QY, 1992, ACTA PHARM SINIC, V13, P416
[9] APOPTOSIS INDUCED BY ANTICANCER DRUGS
HICKMAN, JA
[J]. CANCER AND METASTASIS REVIEWS, 1992, 11 (02) : 121 - 139
[10] PROTEIN OR RNA-SYNTHESIS INHIBITION INDUCES APOPTOSIS OF MATURE HUMAN CD4+ T-CELL BLASTS
MARTIN, SJ
[J]. IMMUNOLOGY LETTERS, 1993, 35 (02) : 125 - 134

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