Anti-Nociceptive and Anti-Inflammatory Effects of Angelicae Dahuricae Radix Through Inhibition of the Expression of Inducible Nitric Oxide Synthase and NO Production

被引：34

作者：

Kang, Ok-Hwa ^{[1
,2
]}

Chae, Hee-Sung ^{[1
,2
]}

Oh, You-Chang ^{[1
,2
]}

Choi, Jang-Gi ^{[1
,2
]}

Lee, Young-Seob ^{[1
,2
]}

Jang, Hye-Jin ^{[1
,2
]}

Kim, Jong-Hak ^{[1
,2
]}

Kim, Youn Chul

Sohn, Dong Hwan

Park, Hyun ^{[3
]}

Kwon, Dong-Yeul ^{[1
,2
]}

机构：

[1] Wonkwang Oriental Med Res Inst, Coll Pharm, Dept Oriental Pharm, Iksan, Jeonbuk, South Korea

[2] Wonkwang Univ, Coll Pharm, Dept Pharm, Iksan 570749, Jeonbuk, South Korea

[3] Wonkwang Univ, Coll Pharm, Dept Parasitol, Iksan 570749, Jeonbuk, South Korea

来源：

AMERICAN JOURNAL OF CHINESE MEDICINE | 2008年 / 36卷 / 05期

关键词：

Angelicae Dahuricae Radix; Anti-Nociceptive; Anti-Inflammatory; Nitric Oxide; Inducible Nitric Oxide Synthase;

D O I：

10.1142/S0192415X0800634X

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

The extract of Angelicae Dahuricae Radix has traditionally been used as an antinoceptive remedy in China. In this study, the methanol extract of Angelicae Dahuricae Radix (MEAD) was evaluated to determine if it has anti-noceptive and anti-inflammatory action. The anti-nociceptive activities of MEAD were evaluated by determining the writhing response and sleeping time, as well as by a formalin test. In addition, the anti-inflammatory activities of MEAD were evaluated by a vascular permeability test as well as by measuring the carrageenan-induced paw edema and conducting a myeloperoxidase (MPO) assay. MEAD (600 and 1200mg/kg) exhibited anti-inflammatory effects on acetic acid-induced vascular permeability, carrageenan-induced paw edema, and MPO activity. Moreover, the results of the formalin test, the acetic acid-induced writhing response and the pentobarbital-induced sleeping time indicated that MEAD had anti-nociceptive effects that occurred in a concentration-dependent manner. To determine the mechanism by which MEAD exerted its effects on the expression of inducible nitric oxide synthase (iNOS) and the production of nitric oxide (NO) by treated murine macrophage RAW 264.7 cells was evaluated. Similar to the ill vivo activities, both the iNOS expression and NO production were significantly suppressed by MEAD in a dose-dependent manner. Furthermore, MEAD inhibited the activating phosphorylation of ERK1/2. These results provide a scientific basis that explains (he mechanism by which Angelicae Dahuricae Radix relieves inflammatory pain.

引用

页码：913 / 928

页数：16

共 32 条

[11] Inhibition of p38 and ERK MAP kinases blocks endotoxin-induced nitric oxide production and differentially modulates cytokine expression [J].