Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis

被引：42
|
作者：
Padilla, S. [1 ,2 ]
Tran, U. C. [3 ]
Jimenez-Hidalgo, M. [1 ,2 ]
Lopez-Martin, J. M. [1 ,2 ]
Martin-Montalvo, A. [1 ,2 ]
Clarke, C. F. [3 ]
Navas, P. [1 ,2 ]
Santos-Ocana, C. [1 ,2 ]
机构：
[1] Univ Pablo Olavide, CSIC, Ctr Andaluz Biol Desarrollo, Seville 41013, Spain
[2] Ctr Biomed Res Rare Dis CIBERER, Seville 41013, Spain
[3] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
来源：
CELLULAR AND MOLECULAR LIFE SCIENCES | 2009年 / 66卷 / 01期
关键词：
Ubiquinone; coenzyme Q; mitochondria; yeast regulation; LIVED CLK-1 MUTANTS; SACCHAROMYCES-CEREVISIAE; UBIQUINONE BIOSYNTHESIS; CAENORHABDITIS-ELEGANS; LIFE-SPAN; FUNCTIONAL EXPRESSION; MULTISUBUNIT COMPLEX; ANTIOXIDANT SYSTEM; GENETIC-EVIDENCE; SIDE-CHAIN;
D O I：
10.1007/s00018-008-8547-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p-Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion from demethoxy-Q (DMQ), a late intermediate. Similar conversion was produced when cells were subjected to oxidative stress conditions. Changes in Q(6)/DMQ(6) ratio were accompanied by changes in COQ7 gene mRNA levels encoding the protein responsible for the DMQ hydroxylation, the penultimate step in Q biosynthesis pathway. Yeast coq null mutant failed to accumulate any Q late biosynthetic intermediate. However, in coq7 mutants the addition of exogenous Q produces the DMQ synthesis. Similar effect was produced by over-expressing ABC1/COQ8. These results support the existence of a biosynthetic complex that allows the DMQ(6) accumulation and suggest that Coq7p is a control point for the Q biosynthesis regulation in yeast.
引用
收藏
页码：173 / 186
页数：14
相关论文
共 30 条
  • [11] Expression of the human atypical kinase ADCK3 rescues coenzyme Q biosynthesis and phosphorylation of Coq polypeptides in yeast coq8 mutants
    Xie, Letian X.
    Hsieh, Edward J.
    Watanabe, Shota
    Allan, Christopher M.
    Chen, Jia Y.
    Tran, UyenPhuong C.
    Clarke, Catherine F.
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2011, 1811 (05): : 348 - 360
  • [12] Involvement of Mitochondrial Ferredoxin and Para-Aminobenzoic Acid in Yeast Coenzyme Q Biosynthesis
    Pierrel, Fabien
    Hamelin, Olivier
    Douki, Thierry
    Kieffer-Jaquinod, Sylvie
    Muehlenhoff, Ulrich
    Ozeir, Mohammad
    Lill, Roland
    Fontecave, Marc
    CHEMISTRY & BIOLOGY, 2010, 17 (05): : 449 - 459
  • [13] Biosynthesis of Coenzyme Q in the Phytopathogen Xanthomonas campestris via a Yeast-Like Pathway
    Zhou, Lian
    Li, Ming
    Wang, Xing-Yu
    Liu, Hao
    Sun, Shuang
    Chen, Haifeng
    Poplawsky, Alan
    He, Ya-Wen
    MOLECULAR PLANT-MICROBE INTERACTIONS, 2019, 32 (02) : 217 - 226
  • [14] Site-directed mutagenesis and structural modeling of Coq10p indicate the presence of a tunnel for coenzyme Q6 binding
    Busso, Cleverson
    Bleicher, Lucas
    Ferreira-Junior, Jose Ribamar
    Barros, Mario H.
    FEBS LETTERS, 2010, 584 (08) : 1609 - 1614
  • [15] Coenzyme Q Biosynthesis: Evidence for a Substrate Access Channel in the FAD-Dependent Monooxygenase Coq6
    Ismail, Alexandre
    Leroux, Vincent
    Smadja, Myriam
    Gonzalez, Lucie
    Lombard, Murielle
    Pierrel, Fabien
    Mellot-Draznieks, Caroline
    Fontecave, Marc
    PLOS COMPUTATIONAL BIOLOGY, 2016, 12 (01)
  • [16] Coq6 Is Responsible for the C4-deamination Reaction in Coenzyme Q Biosynthesis in Saccharomyces cerevisiae
    Ozeir, Mohammad
    Pelosi, Ludovic
    Ismail, Alexandre
    Mellot-Draznieks, Caroline
    Fontecave, Marc
    Pierrel, Fabien
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2015, 290 (40) : 24140 - 24151
  • [17] Activation of Coq6p, a FAD Monooxygenase Involved in Coenzyme Q Biosynthesis, by Adrenodoxin Reductase/Ferredoxin
    Gonzalez, Lucie
    Vo, Samuel Chau-Duy Tam
    Faivre, Bruno
    Pierrel, Fabien
    Fontecave, Marc
    Hamdane, Djemel
    Lombard, Murielle
    CHEMBIOCHEM, 2024, 25 (05)
  • [18] ubiI, a New Gene in Escherichia coli Coenzyme Q Biosynthesis, Is Involved in Aerobic C5-hydroxylation
    Chehade, Mahmoud Hajj
    Loiseau, Laurent
    Lombard, Murielle
    Pecqueur, Ludovic
    Ismail, Alexandre
    Smadja, Myriam
    Golinelli-Pimpaneau, Beatrice
    Mellot-Draznieks, Caroline
    Hamelin, Olivier
    Aussel, Laurent
    Kieffer-Jaquinod, Sylvie
    Labessan, Natty
    Barras, Frederic
    Fontecave, Marc
    Pierrel, Fabien
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (27) : 20085 - 20092
  • [19] The Phosphatase Ptc7 Induces Coenzyme Q Biosynthesis by Activating the Hydroxylase Coq7 in Yeast
    Martin-Montalvo, Alejandro
    Gonzalez-Mariscal, Isabel
    Pomares-Viciana, Teresa
    Padilla-Lopez, Sergio
    Ballesteros, Manuel
    Vazquez-Fonseca, Luis
    Gandolfo, Pablo
    Brautigan, David L.
    Navas, Placido
    Santos-Ocana, Carlos
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2013, 288 (39) : 28126 - 28137
  • [20] Coenzyme Q10 and Q9 Contents in 6 Commercial Vegetable Oils and Their Average Daily Intakes in Korea
    Pyo, Young-Hee
    FOOD SCIENCE AND BIOTECHNOLOGY, 2010, 19 (03) : 837 - 841
123