Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis

被引：42
|
作者：
Padilla, S. [1 ,2 ]
Tran, U. C. [3 ]
Jimenez-Hidalgo, M. [1 ,2 ]
Lopez-Martin, J. M. [1 ,2 ]
Martin-Montalvo, A. [1 ,2 ]
Clarke, C. F. [3 ]
Navas, P. [1 ,2 ]
Santos-Ocana, C. [1 ,2 ]
机构：
[1] Univ Pablo Olavide, CSIC, Ctr Andaluz Biol Desarrollo, Seville 41013, Spain
[2] Ctr Biomed Res Rare Dis CIBERER, Seville 41013, Spain
[3] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
来源：
CELLULAR AND MOLECULAR LIFE SCIENCES | 2009年 / 66卷 / 01期
关键词：
Ubiquinone; coenzyme Q; mitochondria; yeast regulation; LIVED CLK-1 MUTANTS; SACCHAROMYCES-CEREVISIAE; UBIQUINONE BIOSYNTHESIS; CAENORHABDITIS-ELEGANS; LIFE-SPAN; FUNCTIONAL EXPRESSION; MULTISUBUNIT COMPLEX; ANTIOXIDANT SYSTEM; GENETIC-EVIDENCE; SIDE-CHAIN;
D O I：
10.1007/s00018-008-8547-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p-Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion from demethoxy-Q (DMQ), a late intermediate. Similar conversion was produced when cells were subjected to oxidative stress conditions. Changes in Q(6)/DMQ(6) ratio were accompanied by changes in COQ7 gene mRNA levels encoding the protein responsible for the DMQ hydroxylation, the penultimate step in Q biosynthesis pathway. Yeast coq null mutant failed to accumulate any Q late biosynthetic intermediate. However, in coq7 mutants the addition of exogenous Q produces the DMQ synthesis. Similar effect was produced by over-expressing ABC1/COQ8. These results support the existence of a biosynthetic complex that allows the DMQ(6) accumulation and suggest that Coq7p is a control point for the Q biosynthesis regulation in yeast.
引用
收藏
页码：173 / 186
页数：14
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