Fertility of cryopreserved ovine semen is determined by sperm velocity

被引：37
作者：
Del Olmo, E. [1 ]
Bisbal, A. [1 ]
Maroto-Morales, A. [1 ]
Garcia-Alvarez, O. [1 ]
Ramon, M. [2 ]
Jimenez-Rabadan, P. [2 ]
Martinez-Pastor, F. [3 ]
Soler, A. J. [1 ]
Garde, J. J. [1 ]
Fernandez-Santos, M. R. [1 ]
机构：
[1] SaBio IREC CSIC UCLM JCCM, Albacete 02071, Spain
[2] Reg Ctr Anim Select & Reprod CERSYRA JCCM, Valdepenas 13300, Spain
[3] Univ Leon, INDEGSAL, ITRA ULE, E-24071 Leon, Spain
来源：
ANIMAL REPRODUCTION SCIENCE | 2013年 / 138卷 / 1-2期
关键词：
Fertility; Cryopreserved semen; Ram; Flow cytometry; CASA; IN-VITRO FERTILIZATION; RED DEER; FIELD FERTILITY; SPERMATOZOA; PREDICTION; POSTTHAW; MOTILITY; CALCIUM; QUALITY;
D O I：
10.1016/j.anireprosci.2013.02.007
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The present study aims to examine the predictive value of some sperm parameters on male fertility. Semen samples from six Manchega rams were collected and cryopreserved. Sperm quality was assessed after thawing and after 2 h of incubation, either in the freezing extender (37 degrees C) or after dilution in Synthetic Oviductal Fluid (SOF) (38 degrees C, 5% CO2), attempting to mimic the physiological conditions of the female reproductive tract. The following sperm parameters were evaluated: motility and kinetic parameters by computer-assisted semen analyzer (CASA), and sperm viability (propidium iodide), mitochondrial membrane potential (JC-1), apoptotic-like membrane changes (YO-PRO-1), acrosomal status (PNA-FITC), and intracellular calcium (fluo-3) by flow cytometiy. Results showed no significant differences between incubation media neither after thawing nor after incubation. There were no significant correlations between fertility and sperm parameters assessed by flow cytometry. However, after incubation in the freezing extender, sperm samples from males with poor fertility yielded less linearity and velocity (P<0.05) as indicated by motility parameters analyzed by CASA. These results indicate that kinematic sperm motility parameters evaluation by CASA might be useful to identify samples with poor fertility. (C) 2013 Elsevier B.V. All rights reserved.
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页码：102 / 109
页数：8
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