High-throughput novel microsatellite marker of faba bean via next generation sequencing

被引：67

作者：

Yang, Tao ^{[1
]}

Bao, Shi-Ying ^{[2
]}

Ford, Rebecca ^{[3
]}

Jia, Teng-Jiao ^{[1
]}

Guan, Jian-Ping ^{[1
]}

He, Yu-Hua ^{[2
]}

Sun, Xue-Lian ^{[1
]}

Jiang, Jun-Ye ^{[1
]}

Hao, Jun-Jie ^{[4
]}

Zhang, Xiao-Yan ^{[4
]}

Zong, Xu-Xiao ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

[2] Yunnan Acad Agr Sci, Inst Grain Crops, Kunming 650205, Peoples R China

[3] Univ Melbourne, Melbourne Sch Land & Environm, Dept Agr & Food Syst, Melbourne, Vic 3010, Australia

[4] Qingdao Acad Agr Sci, Qingdao 266100, Peoples R China

来源：

BMC GENOMICS | 2012年 / 13卷

基金：

中国国家自然科学基金;

关键词：

Microsatellite markers; Next generation sequencing; Marker development; Vicia faba L;

D O I：

10.1186/1471-2164-13-602

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Faba bean (Vicia faba L.) is an important food legume crop, grown for human consumption globally including in China, Turkey, Egypt and Ethiopia. Although genetic gain has been made through conventional selection and breeding efforts, this could be substantially improved through the application of molecular methods. For this, a set of reliable molecular markers representative of the entire genome is required. Results: A library with 125,559 putative SSR sequences was constructed and characterized for repeat type and length from a mixed genome of 247 spring and winter sown faba bean genotypes using 454 sequencing. A suit of 28,503 primer pair sequences were designed and 150 were randomly selected for validation. Of these, 94 produced reproducible amplicons that were polymorphic among 32 faba bean genotypes selected from diverse geographical locations. The number of alleles per locus ranged from 2 to 8, the expected heterozygocities ranged from 0.0000 to 1.0000, and the observed heterozygosities ranged from 0.0908 to 0.8410. The validation by UPGMA cluster analysis of 32 genotypes based on Nei's genetic distance, showed high quality and effectiveness of those novel SSR markers developed via next generation sequencing technology. Conclusions: Large scale SSR marker development was successfully achieved using next generation sequencing of the V. faba genome. These novel markers are valuable for constructing genetic linkage maps, future QTL mapping, and marker-assisted trait selection in faba bean breeding efforts.

引用

页数：11

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