Target binding improves relaxivity in aptamer-gadolinium conjugates

被引:13
作者
Bernard, Elyse D. [1 ]
Beking, Michael A. [1 ]
Rajamanickam, Karunanithi [2 ]
Tsai, Eve C. [2 ]
DeRosa, Maria C. [1 ]
机构
[1] Carleton Univ, Ottawa Carleton Chem Inst, Dept Chem, Ottawa, ON K1S 5B6, Canada
[2] Ottawa Hosp, Neurosci Unit C2, Ottawa Hosp Res Inst, Ottawa, ON K1Y 4E9, Canada
来源
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY | 2012年 / 17卷 / 08期
基金
加拿大自然科学与工程研究理事会;
关键词
DNA aptamers; Aptamer-gadolinium conjugates; Magnetic resonance imaging; DTPA; Targeted contrast agents; MRI CONTRAST AGENTS; NEPHROGENIC SYSTEMIC FIBROSIS; IN-VITRO; PARAMETERS; COMPLEXES; SELECTION; NMR; TRANSMETALATION; STABILITY; MOLECULES;
D O I
10.1007/s00775-012-0930-z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
MRI contrast agents (CA) have been heavily used over the past several decades to enhance the diagnostic value of the obtained images. From a design perspective, two avenues to improve the efficacy of contrast agents are readily evident: optimization of magnetic properties of the CA, and optimization of the pharmacokinetics and distribution of the CA in the patient. Contrast agents consisting of DNA aptamer-gadolinium(III) conjugates provide a single system in which these factors can be addressed simultaneously. In this proof-of-concept study, the 15mer thrombin aptamer was conjugated to diethylenetriaminepentaacetic (DTPA) dianhydride to form a monoamide derivative of the linear open-chain chelate present in the commonly used contrast agent Magnevist(A (R)). The stability of the conjugated DNA aptamer-DTPA-Gd(III) chelate in a transmetallation study using Zn(II) was found to be similar to that reported for DTPA-Gd(III). Relaxivity enhancements of 35 +/- A 4 and 20 +/- A 1 % were observed in the presence of thrombin compared to a control protein at fields of 9.4 and 1.5 T, respectively. The inclusion of spacers between the aptamer and the DTPA to eliminate possible steric effects was also investigated but not found to improve the relaxation enhancement achieved in comparison to the unaltered aptamer conjugate.
引用
收藏
页码:1159 / 1175
页数:17
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