Spatial orientation mapping of fibers using polarization-sensitive second harmonic generation microscopy

被引：9
作者：
Hovhannisyan, Vladimir A. [2 ]
Hu, Po-Sheng [2 ]
Tan, Hsing-Yuan [3 ]
Chen, Shean-Jen [1 ]
Dong, Chen-Yuan [2 ,4 ]
机构：
[1] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 701, Taiwan
[2] Natl Taiwan Univ, Dept Phys, Taipei 106, Taiwan
[3] Chang Gung Mem Hosp, Dept Ophthalmol, Tao Yuan 333, Taiwan
[4] Natl Taiwan Univ, Ctr Quantum Sci & Engn, Taipei 106, Taiwan
来源：
JOURNAL OF BIOPHOTONICS | 2012年 / 5卷 / 10期
关键词：
polarization-resolved microscopy; second harmonic generation; three-dimensional image processing; collagen; fiber orientation; COLLAGEN;
D O I：
10.1002/jbio.201100123
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this work, we present a non-invasive approach to determine azimuth and elevation angles of collagen fibers capable of generating second harmonic signal. The azimuth angle was determined using the minimum of second harmonic generation (SHG) signal while rotating the plane of polarization of excitation light. The elevation angle was estimated from the ratio of the minimal SHG intensity to the intensity when laser polarization and fiber directions were parallel to each other using experimentally determined calibration curve. Pixel-resolution images of collagen fiber spatial orientation in tendon from bovine leg, chicken leg, and chicken skin were acquired using our approach of SHG polarization-resolved microscopy. ((c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
引用
收藏
页码：768 / 776
页数：9
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