A colorimetric DNA diagnostic method for falciparum malaria and vivax malaria: A field trial in the Solomon Islands

被引：10

作者：

Arai, M

Kunisada, K

Kim, HS

Miyake, H

Mizukoshi, C

Kakutani, T

Yamane, A

Nakagami, S

Kawai, S

Nakano, H

Kawamoto, F

Wataya, Y

机构：

[1] OKAYAMA UNIV,FAC PHARMACEUT SCI,TSUSHIMA,OSAKA 700,JAPAN

[2] OKAYAMA UNIV,SCH MED,DEPT PARASITOL,SHIKATA,OKAYAMA 700,JAPAN

[3] WAKUNAGA PHARMACEUT CO LTD,BIOTECHNOL RES INST,TAKATA,HIROSHIMA 72964,JAPAN

[4] NAGOYA UNIV,SCH MED,DEPT MED ZOOL,SHOWA KU,NAGOYA,AICHI 466,JAPAN

来源：

NUCLEOSIDES & NUCLEOTIDES | 1996年 / 15卷 / 1-3期

关键词：

D O I：

10.1080/07328319608002417

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a colorimetric assay, ''microtiter plate-hybridization'', for the detection of malaria parasites Plasmodium falciparum and P. vivax in human blood, in which the target DNA sequences (18S small subunit ribosomal RNA gene) amplified by polymerase chain reaction (PCR) are hybridized with the species-specific probes immobilized on a microtiter well, This assay system was tested in Guadalcanal, Solomon Islands, where malaria is highly endemic. We obtained blood samples by finger puncture from 130 asymptomatic donors. Among the 130 samples, 30 (23 %) were P. falciparum positive, 28 (22 %) were P. vivax positive, and 8 (6 %) were mixed infections, The results of our DNA diagnostic method showed good correlation with those of acridine orange microscopy.

引用

页码：719 / 731

页数：13

共 32 条

[1] DNA DIAGNOSIS OF OVALE MALARIA AND MALARIAE MALARIA USING MICROTITER PLATE-HYBRIDIZATION [J].