Genetic characterization of Cylindrospermopsis raciborskii (Cyanobacteria) isolates from diverse geographic origins based on nifH and cpcBA-IGS nucleotide sequence analysis

被引：73
作者：
Dyble, J [1 ]
Paerl, HW
Neilan, BA
机构：
[1] Univ N Carolina, Inst Marine Sci, Morehead City, NC 28557 USA
[2] Univ New S Wales, Sch Microbiol & Immunol, Sydney, NSW 2052, Australia
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2002年 / 68卷 / 05期
关键词：
D O I：
10.1128/AEM.68.5.2567-2571.2002
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Isolates of the toxic, N-2-fixing species Cylindrospermopsis raciborskii from various geographic locations were analyzed with respect to their genetic diversity based on the nifH and cpcBA-IGS genes. Gene sequences clustered according to their geographic origin, with the nifH sequences separating into European, Australian, and American groups and the cpcBA-IGS sequences separating into American and European or Australian groups. PCR primers for both genes were designed to exclusively amplify DNA from Cylindrospermopsis species, and an additional primer set for cpcBA-IGS was designed to specifically amplify the American C. raciborskii strains.
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页码：2567 / 2571
页数：5
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