Novel Parallelized Quadrupole/Linear Ion Trap/Orbitrap Tribrid Mass Spectrometer Improving Proteome Coverage and Peptide Identification Rates

被引：178

作者：

Senko, Michael W. ^{[1
]}

Remes, Philip M. ^{[1
]}

Canterbury, Jesse D. ^{[1
]}

Mathur, Raman ^{[1
]}

Song, Qingyu ^{[1
]}

Eliuk, Shannon M. ^{[1
]}

Mullen, Chris ^{[1
]}

Earley, Lee ^{[1
]}

Hardman, Mark ^{[1
]}

Blethrow, Justin D. ^{[1
]}

Bui, Huy ^{[1
]}

Specht, August ^{[1
]}

Lange, Oliver ^{[2
]}

Denisov, Eduard ^{[2
]}

Makarov, Alexander ^{[2
]}

Horning, Stevan ^{[2
]}

Zabrouskov, Vlad ^{[1
]}

机构：

[1] Thermo Fisher Sci, San Jose, CA 95134 USA

[2] Thermo Fisher Sci Bremen GmbH, D-28199 Bremen, Germany

来源：

ANALYTICAL CHEMISTRY | 2013年 / 85卷 / 24期

关键词：

ELECTRON-TRANSFER DISSOCIATION; PERFORMANCE EVALUATION; PEAK-CAPACITY; QUANTIFICATION; NUMBER;

D O I：

10.1021/ac403115c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Proteome coverage and peptide identification rates have historically advanced in line with improvements to the detection limits and acquisition rate of the mass spectrometer. For a linear ion trap/Orbitrap hybrid, the acquisition rate has been limited primarily by the duration of the ion accumulation and analysis steps. It is shown here that the spectral acquisition rate can be significantly improved through extensive parallelization of the acquisition process using a novel mass spectrometer incorporating quadrupole, Orbitrap, and linear trap analyzers. Further, these improvements to the acquisition rate continue to enhance proteome coverage and general experimental throughput.

引用

页码：11710 / 11714

页数：5

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