Study of cerebrovascular reserve capacity by magnetic resonance perfusion weighted imaging and photoacoustic imaging

The aim of this work was to assess the feasibility of photoacoustic imaging (PAI) and MR imaging for evaluating the cerebrovascular reserve capacity (CVRC) in animal models. Wistar-Kyoto (WKY) rats and spontaneous hypertensive rats (STIR) were used for MRI. BALB/c mice were used for PAT. MR perfusion weighted imaging (PWI) was performed oil a 1.5-T whole-body MR system before and after oral administration of acetazolamide (ACZ). The region of interest (ROI) was chosen in the bilateral frontal lobe for measuring regional cerebral blood flow (rCBF), regional cerebral blood volume (rCBV) and mean transit time (MTT). The vessel diameters of the superficial layer of the cortex were measured by PAT in the resting and ACZ-activated mice. The results Showed that there was a statistical difference between the resting and ACZ-activated animals in vessel diameter, rCBV and rCBF values. The increments in rCBV and rCBF of WKY rats between resting and ACZ test states were significantly higher than that of SIIR. The pathological findings of small arterial walls and lumen of the brain were also different between WKY and STIR rats, The diameters of blood vessels in the superficial layer of the brain measured by PAT were enlarged after the ACZ tolerance test. This result was also observed in the MRI CBV map, where the signal of the vessel in the superficial layer of the cortex became redder after the ACZ stimulation, Suggesting the increase of blood flow. It can be concluded that MR PWI and PAI combined with the ACZ test might be Useful in evaluating the CVRC and revealing the pathologic changes in cerebral vessels. (C) 2009 Elsevier Inc. All rights reserved.