Production of Inflammatory Cytokines and Nitric Oxide by Human Mast Cells Incubated with Toxoplasma gondii Lysate

被引：5

作者：

Park, Eun-Ah ^{[1
,2
]}

Hang, Ik-Hwan ^{[3
]}

Kim, Jung-Hyun ^{[3
]}

Park, Soon-Jung ^{[1
,2
]}

Ryu, Jae-Sook ^{[3
]}

Ahn, Myoung-Hee ^{[3
]}

机构：

[1] Yonsei Univ, Coll Med, Dept Environm Med Biol, Seoul 03722, South Korea

[2] Yonsei Univ, Coll Med, Inst Trop Med, Brain Korea PLUS Project Med Sci 21, Seoul 03722, South Korea

[3] Hanyang Univ, Coll Med, Dept Environm Biol & Med Parasitol, Seoul 04763, South Korea

来源：

KOREAN JOURNAL OF PARASITOLOGY | 2019年 / 57卷 / 02期

关键词：

Toxoplasma gondii; mast cell; cytokine; nitric oxide; EXPRESSION; INFECTION; SYNTHASE;

D O I：

10.3347/kjp.2019.57.2.201

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The roles of mast cells in allergic diseases and helminth infections are well known. However, the roles of mast cells in T. gondii infection is poorly understood. This study was focused on the production of pro-inflammatory cytokines (TNF-alpha, IL-4), chemokines (CXCL8, MCP-1) and nitric oxide (NO) by mast cells in response to soluble lysate of T. gondii tachyzoites. Production of CXCL8 (IL-8), MCP-1, TNF-alpha and IL-4 were measured by RT-PCR and ELISA. Western blot were used for detection of CXCR-1 and CXCR2. Our results showed that T. gondii lysates triggered mast cells to release CXCL8, MCP-1, TNF-alpha, IL-4 and to produce NO. This suggests that mast cells play an important role in inflammatory responses to T. gondii.

引用

页码：201 / 206

页数：6

共 23 条

[1] Dendritic Cells Expressing Plasmacytoid Marker PDCA-1 Are Trojan Horses during Toxoplasma gondii Infection
Bierly, Allison L.
Shufesky, William J.
Sukhumavasi, Woraporn
Morelli, Adrian E.
Denkers, Eric Y.
[J]. JOURNAL OF IMMUNOLOGY, 2008, 181 (12) : 8485 - 8491
[2] Mast cells are crucial in the resistance against Toxoplasma gondii oral infection
Cruz, Aline
Mendes, Erica Araujo
Melo de Andrade, Marcus Vinicius
do Nascimento, Vanuza Chagas
Cartelle, Christiane T.
Arantes, Rosa M. E.
da Cunha Melo, Jose Renan
Gazzinelli, Ricardo Tostes
Ropert, Catherine
[J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 2014, 44 (10) : 2949 - 2954
[3] Denney CF, 1999, INFECT IMMUN, V67, P1547
[4] Dlugonska Henryka, 2014, Annals of Parasitology, V60, P235
[5] Toxoplasma gondii and mast cell interactions in vivo and in vitro:: experimental infection approaches in Calomys callosus (Rodentia, Cricetidae)
Ferreira, GLS
Mineo, JR
Oliveira, JG
Ferro, AMV
Souza, MA
Santos, AAD
[J]. MICROBES AND INFECTION, 2004, 6 (02) : 172 - 181
[6] Cytokine regulation of immunopathology in toxoplasmosis
Gaddi, Pamela J.
Yap, George S.
[J]. IMMUNOLOGY AND CELL BIOLOGY, 2007, 85 (02) : 155 - 159
[7] Henderson WR, 1998, J INFECT DIS, V177, P1437, DOI 10.1086/517833
[8] Huang B, 2013, PLOS ONE, V8, P10
[9] HISTAMINE AND TNF-α RELEASE BY RAT PERITONEAL MAST CELLS STIMULATED WITH TRICHOMONAS VAGINALIS
Im, S. J.
Ahn, M. N.
Han, I. H.
Song, H. O.
Kim, Y. S.
Kim, H. M.
Ryu, J. S.
[J]. PARASITE, 2011, 18 (01) : 49 - 55
[10] Modelling parasite dissemination: host cell subversion and immune evasion by Toxoplasma gondii
Lambert, Henrik
Barragan, Antonio
[J]. CELLULAR MICROBIOLOGY, 2010, 12 (03) : 292 - 300

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